Exploring the Biochemical Profile of <i>Beta vulgaris</i> L.: A Comparative Study of Beetroots and Swiss Chard

In this study, leaves and roots from three beetroot cultivars (cv. Albina Vereduna (white roots), cv. Burpee’s Golden (golden roots), and cv. Pablo F1 (red roots)), as well as Swiss chard leaves (also known as “rhubarb chard”, or <i>Beta vulgaris</i> subsp. <i>cicla</i> var....

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Main Authors: Daiana Almeida, Spyridon A. Petropoulos, Tayse F. F. da Silveira, Tânia C. S. P. Pires, Isabel C. F. R. Ferreira, Ângela Fernandes, Lillian Barros
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/4/591
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author Daiana Almeida
Spyridon A. Petropoulos
Tayse F. F. da Silveira
Tânia C. S. P. Pires
Isabel C. F. R. Ferreira
Ângela Fernandes
Lillian Barros
author_facet Daiana Almeida
Spyridon A. Petropoulos
Tayse F. F. da Silveira
Tânia C. S. P. Pires
Isabel C. F. R. Ferreira
Ângela Fernandes
Lillian Barros
author_sort Daiana Almeida
collection DOAJ
description In this study, leaves and roots from three beetroot cultivars (cv. Albina Vereduna (white roots), cv. Burpee’s Golden (golden roots), and cv. Pablo F1 (red roots)), as well as Swiss chard leaves (also known as “rhubarb chard”, or <i>Beta vulgaris</i> subsp. <i>cicla</i> var. <i>flavescens</i>) were evaluated in terms of their chemical profile and bioactive properties. Roots were characterized by high carbohydrate content, which also contributed to greater energy values. In contrast, fibers were the predominant macronutrient in leaves, followed by carbohydrates. In both leaves and roots, the most abundant organic acids were quinic and oxalic, while the major free sugar was sucrose. The profile of fatty acid varied between the studied plant parts, with saturated fatty acids prevailing in root samples, while leaves exhibited higher levels of polyunsaturated fatty acids. Regarding phenolic composition, a total of 19 compounds were tentatively identified in leaves (including derivatives of vitexin, isorhamnetin, quercetin, and ferulic, sinapic, and <i>p</i>-coumaric acids), while the roots exhibited a less diverse composition, with a total of eight compounds identified (e.g., derivatives of ferulic, sinapic, <i>p</i>-coumaric and caffeic acids). A total of eight betalains were also identified, out of which seven were classified as betacyanins and one as betaxanthin. The leaves of Swiss chard presented compounds from both classes, while the roots and leaves of cv. Pablo F1 were characterized only by the presence of betacyanins, and those of cv. Burpee’s Golden only by betaxanthin. All samples exhibited relevant activity against <i>Y. enterocolitica</i>, <i>L. monocytogenes</i>, and <i>S. aureus</i>, although leaf samples demonstrated better antioxidant capacity. In conclusion, beetroot leaves outperformed their corresponding roots in terms of chemical composition, antioxidant, and antimicrobial activity, suggesting their high potential as nutrient-rich and functional ingredients in a diverse and well-balanced diet.
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spelling doaj-art-e6c284f9c7fd411280bd9b3fc6e4da472025-08-20T03:12:06ZengMDPI AGPlants2223-77472025-02-0114459110.3390/plants14040591Exploring the Biochemical Profile of <i>Beta vulgaris</i> L.: A Comparative Study of Beetroots and Swiss ChardDaiana Almeida0Spyridon A. Petropoulos1Tayse F. F. da Silveira2Tânia C. S. P. Pires3Isabel C. F. R. Ferreira4Ângela Fernandes5Lillian Barros6Centro de Investigação de Montanha, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalDepartment of Agriculture Crop Production and Rural Environment, University of Thessaly, Fytokou Street, 38446 Volos, GreeceCentro de Investigação de Montanha, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalCentro de Investigação de Montanha, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalCentro de Investigação de Montanha, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalCentro de Investigação de Montanha, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalCentro de Investigação de Montanha, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalIn this study, leaves and roots from three beetroot cultivars (cv. Albina Vereduna (white roots), cv. Burpee’s Golden (golden roots), and cv. Pablo F1 (red roots)), as well as Swiss chard leaves (also known as “rhubarb chard”, or <i>Beta vulgaris</i> subsp. <i>cicla</i> var. <i>flavescens</i>) were evaluated in terms of their chemical profile and bioactive properties. Roots were characterized by high carbohydrate content, which also contributed to greater energy values. In contrast, fibers were the predominant macronutrient in leaves, followed by carbohydrates. In both leaves and roots, the most abundant organic acids were quinic and oxalic, while the major free sugar was sucrose. The profile of fatty acid varied between the studied plant parts, with saturated fatty acids prevailing in root samples, while leaves exhibited higher levels of polyunsaturated fatty acids. Regarding phenolic composition, a total of 19 compounds were tentatively identified in leaves (including derivatives of vitexin, isorhamnetin, quercetin, and ferulic, sinapic, and <i>p</i>-coumaric acids), while the roots exhibited a less diverse composition, with a total of eight compounds identified (e.g., derivatives of ferulic, sinapic, <i>p</i>-coumaric and caffeic acids). A total of eight betalains were also identified, out of which seven were classified as betacyanins and one as betaxanthin. The leaves of Swiss chard presented compounds from both classes, while the roots and leaves of cv. Pablo F1 were characterized only by the presence of betacyanins, and those of cv. Burpee’s Golden only by betaxanthin. All samples exhibited relevant activity against <i>Y. enterocolitica</i>, <i>L. monocytogenes</i>, and <i>S. aureus</i>, although leaf samples demonstrated better antioxidant capacity. In conclusion, beetroot leaves outperformed their corresponding roots in terms of chemical composition, antioxidant, and antimicrobial activity, suggesting their high potential as nutrient-rich and functional ingredients in a diverse and well-balanced diet.https://www.mdpi.com/2223-7747/14/4/591chemical compositionbioactive propertiesbetalainsphenolic compositionantimicrobial propertiesantioxidant activity
spellingShingle Daiana Almeida
Spyridon A. Petropoulos
Tayse F. F. da Silveira
Tânia C. S. P. Pires
Isabel C. F. R. Ferreira
Ângela Fernandes
Lillian Barros
Exploring the Biochemical Profile of <i>Beta vulgaris</i> L.: A Comparative Study of Beetroots and Swiss Chard
Plants
chemical composition
bioactive properties
betalains
phenolic composition
antimicrobial properties
antioxidant activity
title Exploring the Biochemical Profile of <i>Beta vulgaris</i> L.: A Comparative Study of Beetroots and Swiss Chard
title_full Exploring the Biochemical Profile of <i>Beta vulgaris</i> L.: A Comparative Study of Beetroots and Swiss Chard
title_fullStr Exploring the Biochemical Profile of <i>Beta vulgaris</i> L.: A Comparative Study of Beetroots and Swiss Chard
title_full_unstemmed Exploring the Biochemical Profile of <i>Beta vulgaris</i> L.: A Comparative Study of Beetroots and Swiss Chard
title_short Exploring the Biochemical Profile of <i>Beta vulgaris</i> L.: A Comparative Study of Beetroots and Swiss Chard
title_sort exploring the biochemical profile of i beta vulgaris i l a comparative study of beetroots and swiss chard
topic chemical composition
bioactive properties
betalains
phenolic composition
antimicrobial properties
antioxidant activity
url https://www.mdpi.com/2223-7747/14/4/591
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