Active Vitamin D Ameliorates Arsenite-Induced Thyroid Dysfunction in Sprague–Dawley Rats by Inhibiting the Toll-like Receptor 4/NF-KappaB-Mediated Inflammatory Response

Arsenic, a well-known environmental endocrine disruptor, exerts interference on the body’s endocrine system. Our previous investigations have demonstrated that chronic exposure to sodium arsenite (NaAsO<sub>2</sub>) can induce thyroid damage and dysfunction in Sprague–Dawley (SD) rats. V...

Full description

Saved in:
Bibliographic Details
Main Authors: Hui Li, Jie Xiang, Qian Song, Ying Jin, Meitong Zhou, Lili Fan, Dapeng Wang
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Toxics
Subjects:
Online Access:https://www.mdpi.com/2305-6304/12/12/887
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850037453544488960
author Hui Li
Jie Xiang
Qian Song
Ying Jin
Meitong Zhou
Lili Fan
Dapeng Wang
author_facet Hui Li
Jie Xiang
Qian Song
Ying Jin
Meitong Zhou
Lili Fan
Dapeng Wang
author_sort Hui Li
collection DOAJ
description Arsenic, a well-known environmental endocrine disruptor, exerts interference on the body’s endocrine system. Our previous investigations have demonstrated that chronic exposure to sodium arsenite (NaAsO<sub>2</sub>) can induce thyroid damage and dysfunction in Sprague–Dawley (SD) rats. Vitamin D (VD) is an indispensable fat-soluble vitamin that plays a crucial role in maintaining thyroid health. In recent years, numerous studies have demonstrated the association between VD deficiency and the development of various thyroid disorders. However, the precise intervention roles and mechanisms of VD in arsenic-induced thyroid injury remain elusive. This study aimed to investigate the intervention effect of VD on NaAsO<sub>2</sub>-induced thyroid dysfunction in SD rats. The results demonstrated that exposure to NaAsO<sub>2</sub> activates the TLR4/NF-κB signaling pathway in thyroid tissue of rats, leading to apoptosis of thyroid cells and subsequent inflammatory damage and disruption of serum thyroid hormone secretion. Supplementation with TAK-242 (a TLR4 inhibitor) and VD effectively inhibits the activation of the TLR4/NF-κB signaling pathway in rat thyroid tissue exposed to NaAsO<sub>2</sub>, thereby reducing the inflammatory damage and dysfunction caused by arsenic exposure. In conclusion, the findings of this study offer innovative insights into the application of VD in the prevention and treatment of thyroid dysfunction caused by arsenic exposure.
format Article
id doaj-art-e6388bcacdad49a18673bb79c5c23a83
institution DOAJ
issn 2305-6304
language English
publishDate 2024-12-01
publisher MDPI AG
record_format Article
series Toxics
spelling doaj-art-e6388bcacdad49a18673bb79c5c23a832025-08-20T02:56:51ZengMDPI AGToxics2305-63042024-12-01121288710.3390/toxics12120887Active Vitamin D Ameliorates Arsenite-Induced Thyroid Dysfunction in Sprague–Dawley Rats by Inhibiting the Toll-like Receptor 4/NF-KappaB-Mediated Inflammatory ResponseHui Li0Jie Xiang1Qian Song2Ying Jin3Meitong Zhou4Lili Fan5Dapeng Wang6Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang 561113, ChinaKey Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang 561113, ChinaKey Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang 561113, ChinaKey Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang 561113, ChinaKey Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang 561113, ChinaKey Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang 561113, ChinaKey Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Public Health, Guizhou Medical University, Guiyang 561113, ChinaArsenic, a well-known environmental endocrine disruptor, exerts interference on the body’s endocrine system. Our previous investigations have demonstrated that chronic exposure to sodium arsenite (NaAsO<sub>2</sub>) can induce thyroid damage and dysfunction in Sprague–Dawley (SD) rats. Vitamin D (VD) is an indispensable fat-soluble vitamin that plays a crucial role in maintaining thyroid health. In recent years, numerous studies have demonstrated the association between VD deficiency and the development of various thyroid disorders. However, the precise intervention roles and mechanisms of VD in arsenic-induced thyroid injury remain elusive. This study aimed to investigate the intervention effect of VD on NaAsO<sub>2</sub>-induced thyroid dysfunction in SD rats. The results demonstrated that exposure to NaAsO<sub>2</sub> activates the TLR4/NF-κB signaling pathway in thyroid tissue of rats, leading to apoptosis of thyroid cells and subsequent inflammatory damage and disruption of serum thyroid hormone secretion. Supplementation with TAK-242 (a TLR4 inhibitor) and VD effectively inhibits the activation of the TLR4/NF-κB signaling pathway in rat thyroid tissue exposed to NaAsO<sub>2</sub>, thereby reducing the inflammatory damage and dysfunction caused by arsenic exposure. In conclusion, the findings of this study offer innovative insights into the application of VD in the prevention and treatment of thyroid dysfunction caused by arsenic exposure.https://www.mdpi.com/2305-6304/12/12/887sodium arsenitethyrotoxicityvitamin Dthyroid hormoneTLR4/NF-κB signaling pathway
spellingShingle Hui Li
Jie Xiang
Qian Song
Ying Jin
Meitong Zhou
Lili Fan
Dapeng Wang
Active Vitamin D Ameliorates Arsenite-Induced Thyroid Dysfunction in Sprague–Dawley Rats by Inhibiting the Toll-like Receptor 4/NF-KappaB-Mediated Inflammatory Response
Toxics
sodium arsenite
thyrotoxicity
vitamin D
thyroid hormone
TLR4/NF-κB signaling pathway
title Active Vitamin D Ameliorates Arsenite-Induced Thyroid Dysfunction in Sprague–Dawley Rats by Inhibiting the Toll-like Receptor 4/NF-KappaB-Mediated Inflammatory Response
title_full Active Vitamin D Ameliorates Arsenite-Induced Thyroid Dysfunction in Sprague–Dawley Rats by Inhibiting the Toll-like Receptor 4/NF-KappaB-Mediated Inflammatory Response
title_fullStr Active Vitamin D Ameliorates Arsenite-Induced Thyroid Dysfunction in Sprague–Dawley Rats by Inhibiting the Toll-like Receptor 4/NF-KappaB-Mediated Inflammatory Response
title_full_unstemmed Active Vitamin D Ameliorates Arsenite-Induced Thyroid Dysfunction in Sprague–Dawley Rats by Inhibiting the Toll-like Receptor 4/NF-KappaB-Mediated Inflammatory Response
title_short Active Vitamin D Ameliorates Arsenite-Induced Thyroid Dysfunction in Sprague–Dawley Rats by Inhibiting the Toll-like Receptor 4/NF-KappaB-Mediated Inflammatory Response
title_sort active vitamin d ameliorates arsenite induced thyroid dysfunction in sprague dawley rats by inhibiting the toll like receptor 4 nf kappab mediated inflammatory response
topic sodium arsenite
thyrotoxicity
vitamin D
thyroid hormone
TLR4/NF-κB signaling pathway
url https://www.mdpi.com/2305-6304/12/12/887
work_keys_str_mv AT huili activevitamindamelioratesarseniteinducedthyroiddysfunctioninspraguedawleyratsbyinhibitingthetolllikereceptor4nfkappabmediatedinflammatoryresponse
AT jiexiang activevitamindamelioratesarseniteinducedthyroiddysfunctioninspraguedawleyratsbyinhibitingthetolllikereceptor4nfkappabmediatedinflammatoryresponse
AT qiansong activevitamindamelioratesarseniteinducedthyroiddysfunctioninspraguedawleyratsbyinhibitingthetolllikereceptor4nfkappabmediatedinflammatoryresponse
AT yingjin activevitamindamelioratesarseniteinducedthyroiddysfunctioninspraguedawleyratsbyinhibitingthetolllikereceptor4nfkappabmediatedinflammatoryresponse
AT meitongzhou activevitamindamelioratesarseniteinducedthyroiddysfunctioninspraguedawleyratsbyinhibitingthetolllikereceptor4nfkappabmediatedinflammatoryresponse
AT lilifan activevitamindamelioratesarseniteinducedthyroiddysfunctioninspraguedawleyratsbyinhibitingthetolllikereceptor4nfkappabmediatedinflammatoryresponse
AT dapengwang activevitamindamelioratesarseniteinducedthyroiddysfunctioninspraguedawleyratsbyinhibitingthetolllikereceptor4nfkappabmediatedinflammatoryresponse