High-throughput phagocytosis assay utilizing a pH-sensitive fluorescent dye
We describe a development of a novel high-throughput phagocytosis assay based on a pH-sensitive cyanine dye, CypHer5E™, which is maximally fluorescent in an acidic environment. This dye is ideally suited for the study of phagocytosis because of the acidic conditions generated in the intracellular ph...
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| Main Authors: | Anton Beletskii, Michael Cooper, Priya Sriraman, Camelia Chiriac, Lihong Zhao, Stewart Abbot, Liming Yu |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2005-12-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/000112001 |
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