High-throughput phagocytosis assay utilizing a pH-sensitive fluorescent dye

We describe a development of a novel high-throughput phagocytosis assay based on a pH-sensitive cyanine dye, CypHer5E™, which is maximally fluorescent in an acidic environment. This dye is ideally suited for the study of phagocytosis because of the acidic conditions generated in the intracellular ph...

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Bibliographic Details
Main Authors: Anton Beletskii, Michael Cooper, Priya Sriraman, Camelia Chiriac, Lihong Zhao, Stewart Abbot, Liming Yu
Format: Article
Language:English
Published: Taylor & Francis Group 2005-12-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/000112001
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