Rational development of FMN-based orthogonal riboswitch that functions in response to specific non-cognate ligand

Abstract Re-engineering natural riboswitches into orthogonal RNA switches by making them functional in response to exogenous ligands but unresponsive to endogenous cognate ligands is a promising yet less explored strategy for developing gene regulatory tools. Herein, we rationally engineer the aptam...

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Bibliographic Details
Main Authors: Divya Ojha, Ambadas B. Rode
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-60722-4
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