Influence of Fermentation of Eurotium cristation on the Flavor Characteristics of Guizhou White Tea

To investigate the effect of fermentation with Eurotium cristatum on the flavor characteristics of Guizhou white tea, this study utilized second-grade white tea from Guizhou as the raw material. Scattered tea was subjected to fermentation with Eurotium cristatum, and the flavor characteristics of tr...

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Main Authors: Jinlong LUO, Zhongying LIU, Shimao FANG, Tuo ZHANG, Yuqiao DAI, Xiaowei YANG, Ting YANG, Mengzhen ZHU, Qiang SHEN
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-06-01
Series:Shipin gongye ke-ji
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Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024090177
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author Jinlong LUO
Zhongying LIU
Shimao FANG
Tuo ZHANG
Yuqiao DAI
Xiaowei YANG
Ting YANG
Mengzhen ZHU
Qiang SHEN
author_facet Jinlong LUO
Zhongying LIU
Shimao FANG
Tuo ZHANG
Yuqiao DAI
Xiaowei YANG
Ting YANG
Mengzhen ZHU
Qiang SHEN
author_sort Jinlong LUO
collection DOAJ
description To investigate the effect of fermentation with Eurotium cristatum on the flavor characteristics of Guizhou white tea, this study utilized second-grade white tea from Guizhou as the raw material. Scattered tea was subjected to fermentation with Eurotium cristatum, and the flavor characteristics of traditional white tea (WT) and white tea fermented with Eurotium cristatum (ECWT) were analyzed using a combination of sensory evaluation, high-performance liquid chromatography (HPLC), headspace solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS), flavor activity value (TAV), odor activity value (OAV), and multivariate statistical methods. The results indicated a significant alteration in the organoleptic quality of WT following fermentation to produce ECWT. Specifically, the color of the tea deepened, while freshness diminished; conversely, sweetness and smoothness were enhanced. The floral aroma was notably reduced, whereas fungal flower notes became more pronounced. Upon fermentation with Eurotium cristatum, a significant variation in the concentrations of various bioactive compounds were observed in the Eurotium cristatum-fermented tea (ECWT) as compared to the traditional white tea (WT). Specifically, the levels of theobromine, epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), epicatechin gallate (ECG), theanine, glutamic acid, aspartic acid, alanine, serine, threonine, valine, leucine, isoleucine, tyrosine, phenylalanine, histidine, lysine, and proline were markedly decreased by 41.68%, 81.12%, 52.48%, 68.22%, 93.07%, 83.64%, 50.00%, 91.67%, 96.49%, 65.79%, 74.36%, 83.33%, 94.79%, 93.75%, 62.50%, 92.31%, and 76.09%, respectively (P<0.05). In contrast, the levels of serine, methionine, and cysteine in ECWT were significantly increased by 2100.00%, 36.25%, and 725.00%, respectively (P<0.05). Notably, the concentrations of caffeine, glycine, and arginine did not show any significant variation (P>0.05). Collectively, the total amino acid content in ECWT was found to be significantly reduced by 86.40% in comparison to traditional white tea (WT) (P<0.05). The key taste compounds of traditional white tea (WT) included caffeine, epigallocatechin (EGC), catechin (C), epicatechin (EC), EGCG, GCG, ECG, theanine, glutamic acid, tyrosine, and methionine. In contrast, the key taste compounds of Eurotium cristatum-fermented tea (ECWT) were caffeine, EGCG, GCG, ECG, theanine, methionine, and cysteine. A total of 74 aroma substances were detected in WT and ECWT, of which 62 were key differential aroma substances and 49 were detected in WT. This included 31 key aroma compounds and 17 essential aroma-causing ingredients. In the case of white tea fermented with Eurotium cristatum (ECWT), 55 aroma substances were identified, comprising 28 key aroma compounds and 16 critical aroma-causing ingredients. The flavor characteristics of white tea underwent significant changes following the fermentation of scattered tea with Eurotium cristatum. The findings of this research can provide valuable theoretical insights for optimizing fermentation processes and developing products derived from Eurotium cristatum scattered tea.
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spelling doaj-art-e5680ac1f97247659d9f1ad52d8dc5a02025-08-20T02:25:26ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-06-014611102010.13386/j.issn1002-0306.20240901772024090177-11Influence of Fermentation of Eurotium cristation on the Flavor Characteristics of Guizhou White TeaJinlong LUO0Zhongying LIU1Shimao FANG2Tuo ZHANG3Yuqiao DAI4Xiaowei YANG5Ting YANG6Mengzhen ZHU7Qiang SHEN8Guizhou Tea Research Institute, Guiyang 550006, ChinaGuizhou Tea Research Institute, Guiyang 550006, ChinaGuizhou Tea Research Institute, Guiyang 550006, ChinaGuizhou Tea Research Institute, Guiyang 550006, ChinaGuizhou Tea Research Institute, Guiyang 550006, ChinaGuizhou Tea Research Institute, Guiyang 550006, ChinaGuizhou Tea Research Institute, Guiyang 550006, ChinaGuizhou Tea Society, Guiyang 550006, ChinaGuizhou Tea Research Institute, Guiyang 550006, ChinaTo investigate the effect of fermentation with Eurotium cristatum on the flavor characteristics of Guizhou white tea, this study utilized second-grade white tea from Guizhou as the raw material. Scattered tea was subjected to fermentation with Eurotium cristatum, and the flavor characteristics of traditional white tea (WT) and white tea fermented with Eurotium cristatum (ECWT) were analyzed using a combination of sensory evaluation, high-performance liquid chromatography (HPLC), headspace solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS), flavor activity value (TAV), odor activity value (OAV), and multivariate statistical methods. The results indicated a significant alteration in the organoleptic quality of WT following fermentation to produce ECWT. Specifically, the color of the tea deepened, while freshness diminished; conversely, sweetness and smoothness were enhanced. The floral aroma was notably reduced, whereas fungal flower notes became more pronounced. Upon fermentation with Eurotium cristatum, a significant variation in the concentrations of various bioactive compounds were observed in the Eurotium cristatum-fermented tea (ECWT) as compared to the traditional white tea (WT). Specifically, the levels of theobromine, epigallocatechin gallate (EGCG), gallocatechin gallate (GCG), epicatechin gallate (ECG), theanine, glutamic acid, aspartic acid, alanine, serine, threonine, valine, leucine, isoleucine, tyrosine, phenylalanine, histidine, lysine, and proline were markedly decreased by 41.68%, 81.12%, 52.48%, 68.22%, 93.07%, 83.64%, 50.00%, 91.67%, 96.49%, 65.79%, 74.36%, 83.33%, 94.79%, 93.75%, 62.50%, 92.31%, and 76.09%, respectively (P<0.05). In contrast, the levels of serine, methionine, and cysteine in ECWT were significantly increased by 2100.00%, 36.25%, and 725.00%, respectively (P<0.05). Notably, the concentrations of caffeine, glycine, and arginine did not show any significant variation (P>0.05). Collectively, the total amino acid content in ECWT was found to be significantly reduced by 86.40% in comparison to traditional white tea (WT) (P<0.05). The key taste compounds of traditional white tea (WT) included caffeine, epigallocatechin (EGC), catechin (C), epicatechin (EC), EGCG, GCG, ECG, theanine, glutamic acid, tyrosine, and methionine. In contrast, the key taste compounds of Eurotium cristatum-fermented tea (ECWT) were caffeine, EGCG, GCG, ECG, theanine, methionine, and cysteine. A total of 74 aroma substances were detected in WT and ECWT, of which 62 were key differential aroma substances and 49 were detected in WT. This included 31 key aroma compounds and 17 essential aroma-causing ingredients. In the case of white tea fermented with Eurotium cristatum (ECWT), 55 aroma substances were identified, comprising 28 key aroma compounds and 16 critical aroma-causing ingredients. The flavor characteristics of white tea underwent significant changes following the fermentation of scattered tea with Eurotium cristatum. The findings of this research can provide valuable theoretical insights for optimizing fermentation processes and developing products derived from Eurotium cristatum scattered tea.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024090177eurotium cristatumscattered tea fermentationguizhou white teaflavor characteristics
spellingShingle Jinlong LUO
Zhongying LIU
Shimao FANG
Tuo ZHANG
Yuqiao DAI
Xiaowei YANG
Ting YANG
Mengzhen ZHU
Qiang SHEN
Influence of Fermentation of Eurotium cristation on the Flavor Characteristics of Guizhou White Tea
Shipin gongye ke-ji
eurotium cristatum
scattered tea fermentation
guizhou white tea
flavor characteristics
title Influence of Fermentation of Eurotium cristation on the Flavor Characteristics of Guizhou White Tea
title_full Influence of Fermentation of Eurotium cristation on the Flavor Characteristics of Guizhou White Tea
title_fullStr Influence of Fermentation of Eurotium cristation on the Flavor Characteristics of Guizhou White Tea
title_full_unstemmed Influence of Fermentation of Eurotium cristation on the Flavor Characteristics of Guizhou White Tea
title_short Influence of Fermentation of Eurotium cristation on the Flavor Characteristics of Guizhou White Tea
title_sort influence of fermentation of eurotium cristation on the flavor characteristics of guizhou white tea
topic eurotium cristatum
scattered tea fermentation
guizhou white tea
flavor characteristics
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024090177
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