Unraveling the Determinants of Protrusion Formation

A computerized morphometric classification technique based on latent factors reveals major protrusion classes: factors 4, 5, and 7. Previous work showed that factor 4 represented filopodia, 5 the distribution of lamellar cytoplasm, and 7 a blunt protrusion. We explore the relationship of focal conta...

Full description

Saved in:
Bibliographic Details
Main Authors: Mita Varghese, Peter Gorsevski, Marilyn L. Cayer, Nancy S. Boudreau, Carol A. Heckman
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:International Journal of Cell Biology
Online Access:http://dx.doi.org/10.1155/2012/402916
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832550176572571648
author Mita Varghese
Peter Gorsevski
Marilyn L. Cayer
Nancy S. Boudreau
Carol A. Heckman
author_facet Mita Varghese
Peter Gorsevski
Marilyn L. Cayer
Nancy S. Boudreau
Carol A. Heckman
author_sort Mita Varghese
collection DOAJ
description A computerized morphometric classification technique based on latent factors reveals major protrusion classes: factors 4, 5, and 7. Previous work showed that factor 4 represented filopodia, 5 the distribution of lamellar cytoplasm, and 7 a blunt protrusion. We explore the relationship of focal contact (FC) characteristics and their integrated actin cables to factors values. The results show that FC maturation/cytoskeletal integration affects factor 5, because FC elongation/integration was correlated with its values. On the contrary, 7 values decreased with maturation, so cable or FC size or their integration must be restricted to form these protrusions. Where integration did occur, the cables showed distinctive size and orientation, as indicated by correlation of 7 values with FC shape. Results obtained with myosin inhibitors support the interpretation that a central, isometric, contractile network puts constraints on both factor 5 and 7 protrusions. We conclude that cells establish functional domains by rearranging the cytoskeleton.
format Article
id doaj-art-e51a7a665ead4425a80fc6075597f64d
institution Kabale University
issn 1687-8876
1687-8884
language English
publishDate 2012-01-01
publisher Wiley
record_format Article
series International Journal of Cell Biology
spelling doaj-art-e51a7a665ead4425a80fc6075597f64d2025-02-03T06:07:35ZengWileyInternational Journal of Cell Biology1687-88761687-88842012-01-01201210.1155/2012/402916402916Unraveling the Determinants of Protrusion FormationMita Varghese0Peter Gorsevski1Marilyn L. Cayer2Nancy S. Boudreau3Carol A. Heckman4Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403-0212, USASchool of Earth, Environment and Society, Bowling Green State University, Bowling Green, OH 43403-0212, USACenter for Microscopy and Microanalysis, Bowling Green State University, Bowling Green, OH 43403-0212, USADepartment of Applied Statistics and Operations Research, Bowling Green State University, Bowling Green, OH 43403-0212, USADepartment of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403-0212, USAA computerized morphometric classification technique based on latent factors reveals major protrusion classes: factors 4, 5, and 7. Previous work showed that factor 4 represented filopodia, 5 the distribution of lamellar cytoplasm, and 7 a blunt protrusion. We explore the relationship of focal contact (FC) characteristics and their integrated actin cables to factors values. The results show that FC maturation/cytoskeletal integration affects factor 5, because FC elongation/integration was correlated with its values. On the contrary, 7 values decreased with maturation, so cable or FC size or their integration must be restricted to form these protrusions. Where integration did occur, the cables showed distinctive size and orientation, as indicated by correlation of 7 values with FC shape. Results obtained with myosin inhibitors support the interpretation that a central, isometric, contractile network puts constraints on both factor 5 and 7 protrusions. We conclude that cells establish functional domains by rearranging the cytoskeleton.http://dx.doi.org/10.1155/2012/402916
spellingShingle Mita Varghese
Peter Gorsevski
Marilyn L. Cayer
Nancy S. Boudreau
Carol A. Heckman
Unraveling the Determinants of Protrusion Formation
International Journal of Cell Biology
title Unraveling the Determinants of Protrusion Formation
title_full Unraveling the Determinants of Protrusion Formation
title_fullStr Unraveling the Determinants of Protrusion Formation
title_full_unstemmed Unraveling the Determinants of Protrusion Formation
title_short Unraveling the Determinants of Protrusion Formation
title_sort unraveling the determinants of protrusion formation
url http://dx.doi.org/10.1155/2012/402916
work_keys_str_mv AT mitavarghese unravelingthedeterminantsofprotrusionformation
AT petergorsevski unravelingthedeterminantsofprotrusionformation
AT marilynlcayer unravelingthedeterminantsofprotrusionformation
AT nancysboudreau unravelingthedeterminantsofprotrusionformation
AT carolaheckman unravelingthedeterminantsofprotrusionformation