Unraveling the Determinants of Protrusion Formation
A computerized morphometric classification technique based on latent factors reveals major protrusion classes: factors 4, 5, and 7. Previous work showed that factor 4 represented filopodia, 5 the distribution of lamellar cytoplasm, and 7 a blunt protrusion. We explore the relationship of focal conta...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Cell Biology |
| Online Access: | http://dx.doi.org/10.1155/2012/402916 |
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| _version_ | 1832550176572571648 |
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| author | Mita Varghese Peter Gorsevski Marilyn L. Cayer Nancy S. Boudreau Carol A. Heckman |
| author_facet | Mita Varghese Peter Gorsevski Marilyn L. Cayer Nancy S. Boudreau Carol A. Heckman |
| author_sort | Mita Varghese |
| collection | DOAJ |
| description | A computerized morphometric classification technique based on latent factors reveals major protrusion classes: factors 4, 5, and 7. Previous work showed that factor 4 represented filopodia, 5 the distribution of lamellar cytoplasm, and 7 a blunt protrusion. We explore the relationship of focal contact (FC) characteristics and their integrated actin cables to factors values. The results show that FC maturation/cytoskeletal integration affects factor 5, because FC elongation/integration was correlated with its values. On the contrary, 7 values decreased with maturation, so cable or FC size or their integration must be restricted to form these protrusions. Where integration did occur, the cables showed distinctive size and orientation, as indicated by correlation of 7 values with FC shape. Results obtained with myosin inhibitors support the interpretation that a central, isometric, contractile network puts constraints on both factor 5 and 7 protrusions. We conclude that cells establish functional domains by rearranging the cytoskeleton. |
| format | Article |
| id | doaj-art-e51a7a665ead4425a80fc6075597f64d |
| institution | Kabale University |
| issn | 1687-8876 1687-8884 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Cell Biology |
| spelling | doaj-art-e51a7a665ead4425a80fc6075597f64d2025-02-03T06:07:35ZengWileyInternational Journal of Cell Biology1687-88761687-88842012-01-01201210.1155/2012/402916402916Unraveling the Determinants of Protrusion FormationMita Varghese0Peter Gorsevski1Marilyn L. Cayer2Nancy S. Boudreau3Carol A. Heckman4Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403-0212, USASchool of Earth, Environment and Society, Bowling Green State University, Bowling Green, OH 43403-0212, USACenter for Microscopy and Microanalysis, Bowling Green State University, Bowling Green, OH 43403-0212, USADepartment of Applied Statistics and Operations Research, Bowling Green State University, Bowling Green, OH 43403-0212, USADepartment of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403-0212, USAA computerized morphometric classification technique based on latent factors reveals major protrusion classes: factors 4, 5, and 7. Previous work showed that factor 4 represented filopodia, 5 the distribution of lamellar cytoplasm, and 7 a blunt protrusion. We explore the relationship of focal contact (FC) characteristics and their integrated actin cables to factors values. The results show that FC maturation/cytoskeletal integration affects factor 5, because FC elongation/integration was correlated with its values. On the contrary, 7 values decreased with maturation, so cable or FC size or their integration must be restricted to form these protrusions. Where integration did occur, the cables showed distinctive size and orientation, as indicated by correlation of 7 values with FC shape. Results obtained with myosin inhibitors support the interpretation that a central, isometric, contractile network puts constraints on both factor 5 and 7 protrusions. We conclude that cells establish functional domains by rearranging the cytoskeleton.http://dx.doi.org/10.1155/2012/402916 |
| spellingShingle | Mita Varghese Peter Gorsevski Marilyn L. Cayer Nancy S. Boudreau Carol A. Heckman Unraveling the Determinants of Protrusion Formation International Journal of Cell Biology |
| title | Unraveling the Determinants of Protrusion Formation |
| title_full | Unraveling the Determinants of Protrusion Formation |
| title_fullStr | Unraveling the Determinants of Protrusion Formation |
| title_full_unstemmed | Unraveling the Determinants of Protrusion Formation |
| title_short | Unraveling the Determinants of Protrusion Formation |
| title_sort | unraveling the determinants of protrusion formation |
| url | http://dx.doi.org/10.1155/2012/402916 |
| work_keys_str_mv | AT mitavarghese unravelingthedeterminantsofprotrusionformation AT petergorsevski unravelingthedeterminantsofprotrusionformation AT marilynlcayer unravelingthedeterminantsofprotrusionformation AT nancysboudreau unravelingthedeterminantsofprotrusionformation AT carolaheckman unravelingthedeterminantsofprotrusionformation |