Transcriptomic and proteomic integrated analysis reveals molecular mechanisms of 3D bioprinted vaginal scaffolds in vaginal regeneration
Abstract 3D Bioprinting technology has been applied to vaginal reconstruction with satisfactory results. Understanding the transcriptome and proteome of regenerated vaginas is essential for knowing how biomaterials and seed cells contribute to vaginal regeneration. There are no reports on the system...
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| Language: | English |
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Nature Portfolio
2025-05-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-00507-3 |
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| author | Xuemei Zhang Jiahua Zheng Liye Zhang Jingkun Zhang Li Feng Lin Zhang Xianghua Huang |
| author_facet | Xuemei Zhang Jiahua Zheng Liye Zhang Jingkun Zhang Li Feng Lin Zhang Xianghua Huang |
| author_sort | Xuemei Zhang |
| collection | DOAJ |
| description | Abstract 3D Bioprinting technology has been applied to vaginal reconstruction with satisfactory results. Understanding the transcriptome and proteome of regenerated vaginas is essential for knowing how biomaterials and seed cells contribute to vaginal regeneration. There are no reports on the systemic analysis of vaginal regeneration transcriptomes or proteomes. This study aims to explore the transcriptomic and proteomic features of vaginal tissue reconstructed with 3D bioprinted scaffolds. The scaffolds were made with biomaterials and bone marrow-derived mesenchymal stem cells (BMSCs) and then transplanted into a rabbit model.rna sequencing was used to analyze the transcriptomes of reconstructed and normal vaginal tissues, identifying 11,956 differentially expressed genes (DEGs). Proteomic analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and data-independent acquisition (DIA) identified 7,363 differentially expressed proteins (DEPs). Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses were performed on DEGs and deps. Results showed that DEGs and deps.were involved in extracellular matrix remodeling, angiogenesis, inflammatory response, epithelialization, and muscle formation. This study shows that 3D bioprinted scaffolds are feasible for vaginal reconstruction and offers new insights into the molecular mechanisms involved. |
| format | Article |
| id | doaj-art-e4d8d160d6544e4f858553116a2ac62f |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-e4d8d160d6544e4f858553116a2ac62f2025-08-20T03:22:08ZengNature PortfolioScientific Reports2045-23222025-05-0115111610.1038/s41598-025-00507-3Transcriptomic and proteomic integrated analysis reveals molecular mechanisms of 3D bioprinted vaginal scaffolds in vaginal regenerationXuemei Zhang0Jiahua Zheng1Liye Zhang2Jingkun Zhang3Li Feng4Lin Zhang5Xianghua Huang6Department of Obstetrics and Gynecology, The Second Hospital of Hebei Medical UniversityDepartment of Obstetrics and Gynecology, The Second Hospital of Hebei Medical UniversityChengde Medical UniversityDepartment of Obstetrics and Gynecology, The Second Hospital of Hebei Medical UniversityDepartment of Gynecology, The Fourth Hospital of ShijiazhuangDepartment of Obstetrics and Gynecology, The Second Hospital of Hebei Medical UniversityDepartment of Obstetrics and Gynecology, The Second Hospital of Hebei Medical UniversityAbstract 3D Bioprinting technology has been applied to vaginal reconstruction with satisfactory results. Understanding the transcriptome and proteome of regenerated vaginas is essential for knowing how biomaterials and seed cells contribute to vaginal regeneration. There are no reports on the systemic analysis of vaginal regeneration transcriptomes or proteomes. This study aims to explore the transcriptomic and proteomic features of vaginal tissue reconstructed with 3D bioprinted scaffolds. The scaffolds were made with biomaterials and bone marrow-derived mesenchymal stem cells (BMSCs) and then transplanted into a rabbit model.rna sequencing was used to analyze the transcriptomes of reconstructed and normal vaginal tissues, identifying 11,956 differentially expressed genes (DEGs). Proteomic analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and data-independent acquisition (DIA) identified 7,363 differentially expressed proteins (DEPs). Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses were performed on DEGs and deps. Results showed that DEGs and deps.were involved in extracellular matrix remodeling, angiogenesis, inflammatory response, epithelialization, and muscle formation. This study shows that 3D bioprinted scaffolds are feasible for vaginal reconstruction and offers new insights into the molecular mechanisms involved.https://doi.org/10.1038/s41598-025-00507-3Vaginal reconstructionBiological scaffoldBone marrow-derived mesenchymal stem cellsProteomicsTranscriptomics |
| spellingShingle | Xuemei Zhang Jiahua Zheng Liye Zhang Jingkun Zhang Li Feng Lin Zhang Xianghua Huang Transcriptomic and proteomic integrated analysis reveals molecular mechanisms of 3D bioprinted vaginal scaffolds in vaginal regeneration Scientific Reports Vaginal reconstruction Biological scaffold Bone marrow-derived mesenchymal stem cells Proteomics Transcriptomics |
| title | Transcriptomic and proteomic integrated analysis reveals molecular mechanisms of 3D bioprinted vaginal scaffolds in vaginal regeneration |
| title_full | Transcriptomic and proteomic integrated analysis reveals molecular mechanisms of 3D bioprinted vaginal scaffolds in vaginal regeneration |
| title_fullStr | Transcriptomic and proteomic integrated analysis reveals molecular mechanisms of 3D bioprinted vaginal scaffolds in vaginal regeneration |
| title_full_unstemmed | Transcriptomic and proteomic integrated analysis reveals molecular mechanisms of 3D bioprinted vaginal scaffolds in vaginal regeneration |
| title_short | Transcriptomic and proteomic integrated analysis reveals molecular mechanisms of 3D bioprinted vaginal scaffolds in vaginal regeneration |
| title_sort | transcriptomic and proteomic integrated analysis reveals molecular mechanisms of 3d bioprinted vaginal scaffolds in vaginal regeneration |
| topic | Vaginal reconstruction Biological scaffold Bone marrow-derived mesenchymal stem cells Proteomics Transcriptomics |
| url | https://doi.org/10.1038/s41598-025-00507-3 |
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