Evaluation of Salt Influence on Sugar Consumption by Suspension Cells Based on Spectroscopic Analysis

The influence of metal salt on sugar consumption by suspension cells in food models constructed by a sugar and salt aqueous solution was investigated based on mid-infrared spectroscopic analysis. The contaminated suspension cells in the food model could be detected using the spectral feature change...

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Main Authors: Ken-ichiro Suehara, Takaharu Kameoka, Atsushi Hashimoto
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Journal of Analytical Methods in Chemistry
Online Access:http://dx.doi.org/10.1155/2013/401718
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author Ken-ichiro Suehara
Takaharu Kameoka
Atsushi Hashimoto
author_facet Ken-ichiro Suehara
Takaharu Kameoka
Atsushi Hashimoto
author_sort Ken-ichiro Suehara
collection DOAJ
description The influence of metal salt on sugar consumption by suspension cells in food models constructed by a sugar and salt aqueous solution was investigated based on mid-infrared spectroscopic analysis. The contaminated suspension cells in the food model could be detected using the spectral feature change that measured the present spectrum subtracted in the initial spectrum. The cells were prepared for growth and although the cell did not grow under the induction period, the cell activation (start of sugar metabolism) was detected on the subtracted spectral behavior before the cell growth. The rough grasp of the spectral change behavior is useful for the high-throughput spectroscopic method to detect the contaminated cell activation. Furthermore, the detailed sugar consumption kinetics of the cells was also investigated based on the spectroscopic method. The kind of added salt in the food model influenced the cell activation and the potassium ions play an important role in the plant cells. The living cells activity in fresh food may act to prevent microbial contamination and to suppress the growth of the contaminated microorganism. Both the simple and detailed analyses based on the spectroscopic method presented in this study might be useful for risk management of food.
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institution Kabale University
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publishDate 2013-01-01
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spelling doaj-art-e49f3726f3a242e182b0b907a982e8c52025-02-03T01:27:29ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/401718401718Evaluation of Salt Influence on Sugar Consumption by Suspension Cells Based on Spectroscopic AnalysisKen-ichiro Suehara0Takaharu Kameoka1Atsushi Hashimoto2Division of Sustainable Resource Sciences, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8507, JapanDivision of Sustainable Resource Sciences, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8507, JapanDivision of Sustainable Resource Sciences, Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho, Tsu, Mie 514-8507, JapanThe influence of metal salt on sugar consumption by suspension cells in food models constructed by a sugar and salt aqueous solution was investigated based on mid-infrared spectroscopic analysis. The contaminated suspension cells in the food model could be detected using the spectral feature change that measured the present spectrum subtracted in the initial spectrum. The cells were prepared for growth and although the cell did not grow under the induction period, the cell activation (start of sugar metabolism) was detected on the subtracted spectral behavior before the cell growth. The rough grasp of the spectral change behavior is useful for the high-throughput spectroscopic method to detect the contaminated cell activation. Furthermore, the detailed sugar consumption kinetics of the cells was also investigated based on the spectroscopic method. The kind of added salt in the food model influenced the cell activation and the potassium ions play an important role in the plant cells. The living cells activity in fresh food may act to prevent microbial contamination and to suppress the growth of the contaminated microorganism. Both the simple and detailed analyses based on the spectroscopic method presented in this study might be useful for risk management of food.http://dx.doi.org/10.1155/2013/401718
spellingShingle Ken-ichiro Suehara
Takaharu Kameoka
Atsushi Hashimoto
Evaluation of Salt Influence on Sugar Consumption by Suspension Cells Based on Spectroscopic Analysis
Journal of Analytical Methods in Chemistry
title Evaluation of Salt Influence on Sugar Consumption by Suspension Cells Based on Spectroscopic Analysis
title_full Evaluation of Salt Influence on Sugar Consumption by Suspension Cells Based on Spectroscopic Analysis
title_fullStr Evaluation of Salt Influence on Sugar Consumption by Suspension Cells Based on Spectroscopic Analysis
title_full_unstemmed Evaluation of Salt Influence on Sugar Consumption by Suspension Cells Based on Spectroscopic Analysis
title_short Evaluation of Salt Influence on Sugar Consumption by Suspension Cells Based on Spectroscopic Analysis
title_sort evaluation of salt influence on sugar consumption by suspension cells based on spectroscopic analysis
url http://dx.doi.org/10.1155/2013/401718
work_keys_str_mv AT kenichirosuehara evaluationofsaltinfluenceonsugarconsumptionbysuspensioncellsbasedonspectroscopicanalysis
AT takaharukameoka evaluationofsaltinfluenceonsugarconsumptionbysuspensioncellsbasedonspectroscopicanalysis
AT atsushihashimoto evaluationofsaltinfluenceonsugarconsumptionbysuspensioncellsbasedonspectroscopicanalysis