Overstretch causes lipid accumulation in vascular smooth muscle cells dependent on NADPH oxidase 1
At the bend and bifurcation of arteries prone to atherosclerosis, pulsatile blood retention may cause overstretch on the tube wall. It has been reported that more than half of the foam cells found in atherosclerotic plaques are derived from vascular smooth muscle cells (VSMCs), but the mechanism is...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | Mechanobiology in Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949907025000178 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849422395629109248 |
|---|---|
| author | Jiazhen Zhang Qinfen Li Suoqi Ding Wei Xu Jilei Su Jingang Cui Yongsheng Ding |
| author_facet | Jiazhen Zhang Qinfen Li Suoqi Ding Wei Xu Jilei Su Jingang Cui Yongsheng Ding |
| author_sort | Jiazhen Zhang |
| collection | DOAJ |
| description | At the bend and bifurcation of arteries prone to atherosclerosis, pulsatile blood retention may cause overstretch on the tube wall. It has been reported that more than half of the foam cells found in atherosclerotic plaques are derived from vascular smooth muscle cells (VSMCs), but the mechanism is not adequately understood. In this work, we used a microfluidic device to apply a cyclic stretch (15 % and 0.05 Hz) on the VSMC for 24 h. The stretch caused a significant increase in the intracellular lipid accumulation, accompanying with the increased NOX1 and CD36 protein expression. On the other hand, inhibition of NOX1 activity, elimination of reactive oxygen species (ROS), or knockdown of NOX1 expression could significantly inhibit intracellular lipid accumulation. In addition, the NOX1 upregulation caused by 15 % stretch was related to the JAK/STAT signaling pathway. Our results reveal a novel mechanism of VSMC foam cell formation caused by the upregulation of NOX1. |
| format | Article |
| id | doaj-art-e38786d8462a4f7f9f1d4da157c72c67 |
| institution | Kabale University |
| issn | 2949-9070 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Mechanobiology in Medicine |
| spelling | doaj-art-e38786d8462a4f7f9f1d4da157c72c672025-08-20T03:31:07ZengElsevierMechanobiology in Medicine2949-90702025-06-013210012910.1016/j.mbm.2025.100129Overstretch causes lipid accumulation in vascular smooth muscle cells dependent on NADPH oxidase 1Jiazhen Zhang0Qinfen Li1Suoqi Ding2Wei Xu3Jilei Su4Jingang Cui5Yongsheng Ding6College of Life Sciences, University of Chinese Academy of Sciences, Beijing 101408, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 101408, ChinaSchool of Chemistry, Beihang University, Beijing, 100191, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 101408, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 101408, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 101408, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing 101408, China; Corresponding author.At the bend and bifurcation of arteries prone to atherosclerosis, pulsatile blood retention may cause overstretch on the tube wall. It has been reported that more than half of the foam cells found in atherosclerotic plaques are derived from vascular smooth muscle cells (VSMCs), but the mechanism is not adequately understood. In this work, we used a microfluidic device to apply a cyclic stretch (15 % and 0.05 Hz) on the VSMC for 24 h. The stretch caused a significant increase in the intracellular lipid accumulation, accompanying with the increased NOX1 and CD36 protein expression. On the other hand, inhibition of NOX1 activity, elimination of reactive oxygen species (ROS), or knockdown of NOX1 expression could significantly inhibit intracellular lipid accumulation. In addition, the NOX1 upregulation caused by 15 % stretch was related to the JAK/STAT signaling pathway. Our results reveal a novel mechanism of VSMC foam cell formation caused by the upregulation of NOX1.http://www.sciencedirect.com/science/article/pii/S2949907025000178OverstretchNADPH oxidase 1Lipid accumulationVascular smooth muscle cellsAtherosclerosis |
| spellingShingle | Jiazhen Zhang Qinfen Li Suoqi Ding Wei Xu Jilei Su Jingang Cui Yongsheng Ding Overstretch causes lipid accumulation in vascular smooth muscle cells dependent on NADPH oxidase 1 Mechanobiology in Medicine Overstretch NADPH oxidase 1 Lipid accumulation Vascular smooth muscle cells Atherosclerosis |
| title | Overstretch causes lipid accumulation in vascular smooth muscle cells dependent on NADPH oxidase 1 |
| title_full | Overstretch causes lipid accumulation in vascular smooth muscle cells dependent on NADPH oxidase 1 |
| title_fullStr | Overstretch causes lipid accumulation in vascular smooth muscle cells dependent on NADPH oxidase 1 |
| title_full_unstemmed | Overstretch causes lipid accumulation in vascular smooth muscle cells dependent on NADPH oxidase 1 |
| title_short | Overstretch causes lipid accumulation in vascular smooth muscle cells dependent on NADPH oxidase 1 |
| title_sort | overstretch causes lipid accumulation in vascular smooth muscle cells dependent on nadph oxidase 1 |
| topic | Overstretch NADPH oxidase 1 Lipid accumulation Vascular smooth muscle cells Atherosclerosis |
| url | http://www.sciencedirect.com/science/article/pii/S2949907025000178 |
| work_keys_str_mv | AT jiazhenzhang overstretchcauseslipidaccumulationinvascularsmoothmusclecellsdependentonnadphoxidase1 AT qinfenli overstretchcauseslipidaccumulationinvascularsmoothmusclecellsdependentonnadphoxidase1 AT suoqiding overstretchcauseslipidaccumulationinvascularsmoothmusclecellsdependentonnadphoxidase1 AT weixu overstretchcauseslipidaccumulationinvascularsmoothmusclecellsdependentonnadphoxidase1 AT jileisu overstretchcauseslipidaccumulationinvascularsmoothmusclecellsdependentonnadphoxidase1 AT jingangcui overstretchcauseslipidaccumulationinvascularsmoothmusclecellsdependentonnadphoxidase1 AT yongshengding overstretchcauseslipidaccumulationinvascularsmoothmusclecellsdependentonnadphoxidase1 |