Engineering novel CRISPRi repressors for highly efficient mammalian gene regulation
Abstract Background CRISPR interference (CRISPRi), the repurposing of the RNA-guided endonuclease dCas9 as a programmable transcriptional repressor, allows highly specific repression (knockdown) of gene expression. CRISPRi platforms can often have incomplete knockdown, performance variability across...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-06-01
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| Series: | Genome Biology |
| Online Access: | https://doi.org/10.1186/s13059-025-03640-4 |
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