Random antimicrobial peptide mixtures as non-antibiotic antimicrobial agents for cultured meat industry
Antibiotics, commonly used in cell culture studies to prevent microbial contamination, cannot be employed in Cultured meat (CM) due to potential residues in the final food products. Hence, there is an urgent need to develop novel and safe non-antibiotic antimicrobial agents. Here, we investigated th...
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Elsevier
2025-06-01
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| Series: | Food Chemistry: Molecular Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666566225000012 |
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| author | Idan Yakir Einav Cohen Sharon Schlesinger Zvi Hayouka |
| author_facet | Idan Yakir Einav Cohen Sharon Schlesinger Zvi Hayouka |
| author_sort | Idan Yakir |
| collection | DOAJ |
| description | Antibiotics, commonly used in cell culture studies to prevent microbial contamination, cannot be employed in Cultured meat (CM) due to potential residues in the final food products. Hence, there is an urgent need to develop novel and safe non-antibiotic antimicrobial agents. Here, we investigated the potential of random antimicrobial peptide mixtures (RPMs) as non-antibiotic antimicrobial agents. RPMs are synthetic peptide cocktails that have previously shown strong and broad antimicrobial activity; however, their use in cell culture media and their effect on mammalian cells have not yet been explored. Here we show that RPMs had no significant impact on mesenchymal stem cells (MSCs) at concentrations that effectively inhibit bacterial growth. RPMs displayed strong bactericidal activity against Gram-positive bacteria, achieving a 6-log reduction of L. monocytogenes in cell culture medium without any cytotoxicity. Additionally, RPMs showed a low occurrence of resistance development with no significant resistance developed in compared with a combination of penicillin and streptomycin. Moreover, LK20 mixture was rapidly digested and a rapid digestion in a simulated digestion model. Our results indicate that RPMs have great potential to serve as safe and effective non antibiotic antimicrobial agents in cultured meat industry. |
| format | Article |
| id | doaj-art-e30a226b8fbf489ebf1bdb248ff0577e |
| institution | Kabale University |
| issn | 2666-5662 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: Molecular Sciences |
| spelling | doaj-art-e30a226b8fbf489ebf1bdb248ff0577e2025-01-25T04:11:26ZengElsevierFood Chemistry: Molecular Sciences2666-56622025-06-0110100240Random antimicrobial peptide mixtures as non-antibiotic antimicrobial agents for cultured meat industryIdan Yakir0Einav Cohen1Sharon Schlesinger2Zvi Hayouka3Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agricultural, Food & Environment, The Hebrew University of Jerusalem, Rehovot, 76100, Israel; Singapore-HUJ Alliance for research and enterprise (SHARE), The Cellular Agriculture (CellAg) Programme, Campus for Research Excellence and Technological Enterprise (CREATE), 138602, SingaporeInstitute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agricultural, Food & Environment, The Hebrew University of Jerusalem, Rehovot, 76100, IsraelDepartment of Animal Sciences, The Robert H. Smith Faculty of Agricultural, Food & Environment, The Hebrew University of Jerusalem, Rehovot, 76100, IsraelInstitute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agricultural, Food & Environment, The Hebrew University of Jerusalem, Rehovot, 76100, Israel; Singapore-HUJ Alliance for research and enterprise (SHARE), The Cellular Agriculture (CellAg) Programme, Campus for Research Excellence and Technological Enterprise (CREATE), 138602, Singapore; Corresponding author at: Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agricultural, Food & Environment, The Hebrew University of Jerusalem, Rehovot, 76100, Israel; Singapore-HUJ Alliance for research and enterprise (SHARE), The Cellular Agriculture (CellAg) Programme, Campus for Research Excellence and Technological Enterprise (CREATE), 138602, SingaporeAntibiotics, commonly used in cell culture studies to prevent microbial contamination, cannot be employed in Cultured meat (CM) due to potential residues in the final food products. Hence, there is an urgent need to develop novel and safe non-antibiotic antimicrobial agents. Here, we investigated the potential of random antimicrobial peptide mixtures (RPMs) as non-antibiotic antimicrobial agents. RPMs are synthetic peptide cocktails that have previously shown strong and broad antimicrobial activity; however, their use in cell culture media and their effect on mammalian cells have not yet been explored. Here we show that RPMs had no significant impact on mesenchymal stem cells (MSCs) at concentrations that effectively inhibit bacterial growth. RPMs displayed strong bactericidal activity against Gram-positive bacteria, achieving a 6-log reduction of L. monocytogenes in cell culture medium without any cytotoxicity. Additionally, RPMs showed a low occurrence of resistance development with no significant resistance developed in compared with a combination of penicillin and streptomycin. Moreover, LK20 mixture was rapidly digested and a rapid digestion in a simulated digestion model. Our results indicate that RPMs have great potential to serve as safe and effective non antibiotic antimicrobial agents in cultured meat industry.http://www.sciencedirect.com/science/article/pii/S2666566225000012Cultured meatNon antibiotic antimicrobial agentAntimicrobial peptideCellular agricultureFood safetyFood preservation |
| spellingShingle | Idan Yakir Einav Cohen Sharon Schlesinger Zvi Hayouka Random antimicrobial peptide mixtures as non-antibiotic antimicrobial agents for cultured meat industry Food Chemistry: Molecular Sciences Cultured meat Non antibiotic antimicrobial agent Antimicrobial peptide Cellular agriculture Food safety Food preservation |
| title | Random antimicrobial peptide mixtures as non-antibiotic antimicrobial agents for cultured meat industry |
| title_full | Random antimicrobial peptide mixtures as non-antibiotic antimicrobial agents for cultured meat industry |
| title_fullStr | Random antimicrobial peptide mixtures as non-antibiotic antimicrobial agents for cultured meat industry |
| title_full_unstemmed | Random antimicrobial peptide mixtures as non-antibiotic antimicrobial agents for cultured meat industry |
| title_short | Random antimicrobial peptide mixtures as non-antibiotic antimicrobial agents for cultured meat industry |
| title_sort | random antimicrobial peptide mixtures as non antibiotic antimicrobial agents for cultured meat industry |
| topic | Cultured meat Non antibiotic antimicrobial agent Antimicrobial peptide Cellular agriculture Food safety Food preservation |
| url | http://www.sciencedirect.com/science/article/pii/S2666566225000012 |
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