METTL14 and FTO mediated m6A modification regulate PCV2 replication by affecting miR-30a-5p maturity
The epigenetic modification of the N6-methyladenosine (m6A) methylation plays an important role in virus infection and replication. However, its role in Porcine circovirus type 2 (PCV2) replication has not been well studied. Here, we demonstrated that m6A modifications are increased in PK-15 cells a...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2023.2232910 |
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| author | Chen Li Xiaoyan Wu Ying Yang Jianli Shi Shuo Wang Jiaxin Li Yao Tian Chang Liu Hong Han Shaojian Xu Xianjie Han Yingru Ma Limei Zheng Jun Li |
| author_facet | Chen Li Xiaoyan Wu Ying Yang Jianli Shi Shuo Wang Jiaxin Li Yao Tian Chang Liu Hong Han Shaojian Xu Xianjie Han Yingru Ma Limei Zheng Jun Li |
| author_sort | Chen Li |
| collection | DOAJ |
| description | The epigenetic modification of the N6-methyladenosine (m6A) methylation plays an important role in virus infection and replication. However, its role in Porcine circovirus type 2 (PCV2) replication has not been well studied. Here, we demonstrated that m6A modifications are increased in PK-15 cells after PCV2 infection. In particular, PCV2 infection could increase the expression of methyltransferase METTL14 and demethylase FTO. Moreover, interfering with METTL14 accumulation reduced the m6A methylation level and virus reproduction, whereas depleting the FTO demethylase enhanced the m6A methylation level and stimulated virus reproduction. Besides, we showed that METTL14 and FTO regulate PCV2 replication by affecting the process of miRNA maturity, especially the miRNA-30a-5p. Taken together, our results demonstrated that the m6A modification positively affects PCV2 replication and the role of m6A modification in the replication mechanism of the PCV2 virus provides a new idea for the prevention and control of the PCV2. |
| format | Article |
| id | doaj-art-e2ee23461ce1457aa5ead7ade7417fa1 |
| institution | Kabale University |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-e2ee23461ce1457aa5ead7ade7417fa12025-08-20T03:32:19ZengTaylor & Francis GroupVirulence2150-55942150-56082023-12-0114110.1080/21505594.2023.2232910METTL14 and FTO mediated m6A modification regulate PCV2 replication by affecting miR-30a-5p maturityChen Li0Xiaoyan Wu1Ying Yang2Jianli Shi3Shuo Wang4Jiaxin Li5Yao Tian6Chang Liu7Hong Han8Shaojian Xu9Xianjie Han10Yingru Ma11Limei Zheng12Jun Li13Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, jinan, P. R. ChinaShandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, jinan, P. R. ChinaCollege of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, jinan, P. R. ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Qingdao, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, ChinaShandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, jinan, P. R. ChinaShandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, jinan, P. R. ChinaShandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, jinan, P. R. ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, ChinaShandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, jinan, P. R. ChinaThe epigenetic modification of the N6-methyladenosine (m6A) methylation plays an important role in virus infection and replication. However, its role in Porcine circovirus type 2 (PCV2) replication has not been well studied. Here, we demonstrated that m6A modifications are increased in PK-15 cells after PCV2 infection. In particular, PCV2 infection could increase the expression of methyltransferase METTL14 and demethylase FTO. Moreover, interfering with METTL14 accumulation reduced the m6A methylation level and virus reproduction, whereas depleting the FTO demethylase enhanced the m6A methylation level and stimulated virus reproduction. Besides, we showed that METTL14 and FTO regulate PCV2 replication by affecting the process of miRNA maturity, especially the miRNA-30a-5p. Taken together, our results demonstrated that the m6A modification positively affects PCV2 replication and the role of m6A modification in the replication mechanism of the PCV2 virus provides a new idea for the prevention and control of the PCV2.https://www.tandfonline.com/doi/10.1080/21505594.2023.2232910Porcine circovirus type 2m6A modificationmiRNA maturityreplication |
| spellingShingle | Chen Li Xiaoyan Wu Ying Yang Jianli Shi Shuo Wang Jiaxin Li Yao Tian Chang Liu Hong Han Shaojian Xu Xianjie Han Yingru Ma Limei Zheng Jun Li METTL14 and FTO mediated m6A modification regulate PCV2 replication by affecting miR-30a-5p maturity Virulence Porcine circovirus type 2 m6A modification miRNA maturity replication |
| title | METTL14 and FTO mediated m6A modification regulate PCV2 replication by affecting miR-30a-5p maturity |
| title_full | METTL14 and FTO mediated m6A modification regulate PCV2 replication by affecting miR-30a-5p maturity |
| title_fullStr | METTL14 and FTO mediated m6A modification regulate PCV2 replication by affecting miR-30a-5p maturity |
| title_full_unstemmed | METTL14 and FTO mediated m6A modification regulate PCV2 replication by affecting miR-30a-5p maturity |
| title_short | METTL14 and FTO mediated m6A modification regulate PCV2 replication by affecting miR-30a-5p maturity |
| title_sort | mettl14 and fto mediated m6a modification regulate pcv2 replication by affecting mir 30a 5p maturity |
| topic | Porcine circovirus type 2 m6A modification miRNA maturity replication |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2023.2232910 |
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