Towards a Survival-Based Cellular Assay for the Selection of Protease Inhibitors in <i>Escherichia coli</i>

Towards a Survival-Based Cellular Assay for the Selection of Protease Inhibitors in <i>Escherichia coli</i>

We describe a method tailored to the in-cell selection of protease inhibitors. In this method, a target protease is co-expressed with a selective substrate, the product of which kills host cells. Therefore, the method can be applied to identify potential inhibitors based on cell host survival when i...

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Bibliographic Details
Main Authors: William Y. Oyadomari, Elizangela A. Carvalho, Gabriel E. Machado, Ana Júlia O. Machado, Gabriel S. Santos, Marcelo Marcondes, Vitor Oliveira
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:BioTech
Subjects:
protease inhibitor
cell-based assay
high-content selection method
high-throughput screening
Online Access:https://www.mdpi.com/2673-6284/14/1/16
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