Novel Quinoline- and Naphthalene-Incorporated Hydrazineylidene–Propenamide Analogues as Antidiabetic Agents: Design, Synthesis, and Computational Studies

<b>Background:</b> Type 2 diabetes has become a significant global health challenge. Numerous drugs have been developed to treat the condition, either as standalone therapies or in combination when glycemic control cannot be achieved with a single medication. As existing treatments often...

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Main Authors: Osama Alharbi, Wael H. Alsaedi, Mosa Alsehli, Saif H. Althagafi, Hussam Y. Alharbi, Yazeed M. Asiri, Ramith Ramu, Mohammed Al-Ghorbani
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Pharmaceuticals
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Online Access:https://www.mdpi.com/1424-8247/17/12/1692
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author Osama Alharbi
Wael H. Alsaedi
Mosa Alsehli
Saif H. Althagafi
Hussam Y. Alharbi
Yazeed M. Asiri
Ramith Ramu
Mohammed Al-Ghorbani
author_facet Osama Alharbi
Wael H. Alsaedi
Mosa Alsehli
Saif H. Althagafi
Hussam Y. Alharbi
Yazeed M. Asiri
Ramith Ramu
Mohammed Al-Ghorbani
author_sort Osama Alharbi
collection DOAJ
description <b>Background:</b> Type 2 diabetes has become a significant global health challenge. Numerous drugs have been developed to treat the condition, either as standalone therapies or in combination when glycemic control cannot be achieved with a single medication. As existing treatments often come with limitations, there is an increasing focus on creating novel therapeutic agents that offer greater efficacy and fewer side effects to better address this widespread issue. <b>Methods</b>: The methylene derivatives <b>3a</b>,<b>b</b> were coupled with phenyl/ethyl isothiocyanate in the basic medium, and dimethyl sulfate was subsequently added. Further, <b>5a</b>–<b>d</b> were reacted with the quinoline/naphthalene hydrazides <b>6a</b>,<b>b</b>. The target compounds <b>7a</b>–<b>g</b> were subjected to the in vitro enzyme inhibition studies on α-glucosidase, α-amylase, and aldose reductase. <b>Results</b>: <b>7g</b> exerted remarkable inhibitory effects on α-glycosidase [Inhibitory Concentration (IC<sub>50</sub>): 20.23 ± 1.10 µg/mL] and α-amylase (17.15 ± 0.30 µg/mL), outperforming acarbose (28.12 ± 0.20 µg/mL for α-glycosidase and 25.42 ± 0.10 µg/mL for α-amylase), and exhibited a strong inhibition action on aldose reductase (12.15 ± 0.24 µg/mL), surpassing quercetin (15.45 ± 0.32 µg/mL) and the other tested compounds. In a computational study, <b>7g</b> demonstrated promising binding affinities (−8.80, −8.91 kcal/mol) with α-glycosidase and α-amylase, compared to acarbose (−10.87, −10.38 kcal/mol) for α-glycosidase and α-amylase. Additionally, <b>7g</b> had strong binding with aldose reductase (−9.20 kcal/mol) in comparison to quercetin (−9.95 kcal/mol). Molecular dynamics (MDs) simulations demonstrated that <b>7g</b> remained stable over a 100 ns simulation period, and the binding free energy estimates remained consistent throughout this time. <b>Conclusions</b>: We reported the modification of quinoline and naphthalene rings to hydrazineylidene–propenamides <b>7a</b>–<b>g</b> using various synthetic approaches. <b>7g</b> emerged as a leading candidate, exhibiting greater inhibition of α-glycosidase, α-amylase, and aldose reductase. These findings underscore their potential as essential molecules for the development of innovative antidiabetic treatments.
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spelling doaj-art-e19137df4f424a129d355c8e7d59101a2025-08-20T02:56:54ZengMDPI AGPharmaceuticals1424-82472024-12-011712169210.3390/ph17121692Novel Quinoline- and Naphthalene-Incorporated Hydrazineylidene–Propenamide Analogues as Antidiabetic Agents: Design, Synthesis, and Computational StudiesOsama Alharbi0Wael H. Alsaedi1Mosa Alsehli2Saif H. Althagafi3Hussam Y. Alharbi4Yazeed M. Asiri5Ramith Ramu6Mohammed Al-Ghorbani7Department of Chemistry, Faculty of Science, Taibah University, Madinah 42353, Saudi ArabiaDepartment of Chemistry, Faculty of Science, Taibah University, Madinah 42353, Saudi ArabiaDepartment of Chemistry, Faculty of Science, Taibah University, Madinah 42353, Saudi ArabiaDepartment of Chemistry, Faculty of Science, Al-Baha University, P.O. Box 1988, Al Baha 65431, Saudi ArabiaDepartment of Chemistry, Faculty of Science, Taibah University, Yanbu 46421, Saudi ArabiaDepartment of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology and Bioinformatics, JSS Academy of Higher Education and Research, Mysuru 570015, IndiaDepartment of Chemistry, Faculty of Science, Taibah University, Madinah 42353, Saudi Arabia<b>Background:</b> Type 2 diabetes has become a significant global health challenge. Numerous drugs have been developed to treat the condition, either as standalone therapies or in combination when glycemic control cannot be achieved with a single medication. As existing treatments often come with limitations, there is an increasing focus on creating novel therapeutic agents that offer greater efficacy and fewer side effects to better address this widespread issue. <b>Methods</b>: The methylene derivatives <b>3a</b>,<b>b</b> were coupled with phenyl/ethyl isothiocyanate in the basic medium, and dimethyl sulfate was subsequently added. Further, <b>5a</b>–<b>d</b> were reacted with the quinoline/naphthalene hydrazides <b>6a</b>,<b>b</b>. The target compounds <b>7a</b>–<b>g</b> were subjected to the in vitro enzyme inhibition studies on α-glucosidase, α-amylase, and aldose reductase. <b>Results</b>: <b>7g</b> exerted remarkable inhibitory effects on α-glycosidase [Inhibitory Concentration (IC<sub>50</sub>): 20.23 ± 1.10 µg/mL] and α-amylase (17.15 ± 0.30 µg/mL), outperforming acarbose (28.12 ± 0.20 µg/mL for α-glycosidase and 25.42 ± 0.10 µg/mL for α-amylase), and exhibited a strong inhibition action on aldose reductase (12.15 ± 0.24 µg/mL), surpassing quercetin (15.45 ± 0.32 µg/mL) and the other tested compounds. In a computational study, <b>7g</b> demonstrated promising binding affinities (−8.80, −8.91 kcal/mol) with α-glycosidase and α-amylase, compared to acarbose (−10.87, −10.38 kcal/mol) for α-glycosidase and α-amylase. Additionally, <b>7g</b> had strong binding with aldose reductase (−9.20 kcal/mol) in comparison to quercetin (−9.95 kcal/mol). Molecular dynamics (MDs) simulations demonstrated that <b>7g</b> remained stable over a 100 ns simulation period, and the binding free energy estimates remained consistent throughout this time. <b>Conclusions</b>: We reported the modification of quinoline and naphthalene rings to hydrazineylidene–propenamides <b>7a</b>–<b>g</b> using various synthetic approaches. <b>7g</b> emerged as a leading candidate, exhibiting greater inhibition of α-glycosidase, α-amylase, and aldose reductase. These findings underscore their potential as essential molecules for the development of innovative antidiabetic treatments.https://www.mdpi.com/1424-8247/17/12/1692quinolinenaphthaleneα-glycosidaseα-amylase and aldose reductase enzyme inhibitionsmolecular docking simulationmolecular dynamics simulation
spellingShingle Osama Alharbi
Wael H. Alsaedi
Mosa Alsehli
Saif H. Althagafi
Hussam Y. Alharbi
Yazeed M. Asiri
Ramith Ramu
Mohammed Al-Ghorbani
Novel Quinoline- and Naphthalene-Incorporated Hydrazineylidene–Propenamide Analogues as Antidiabetic Agents: Design, Synthesis, and Computational Studies
Pharmaceuticals
quinoline
naphthalene
α-glycosidase
α-amylase and aldose reductase enzyme inhibitions
molecular docking simulation
molecular dynamics simulation
title Novel Quinoline- and Naphthalene-Incorporated Hydrazineylidene–Propenamide Analogues as Antidiabetic Agents: Design, Synthesis, and Computational Studies
title_full Novel Quinoline- and Naphthalene-Incorporated Hydrazineylidene–Propenamide Analogues as Antidiabetic Agents: Design, Synthesis, and Computational Studies
title_fullStr Novel Quinoline- and Naphthalene-Incorporated Hydrazineylidene–Propenamide Analogues as Antidiabetic Agents: Design, Synthesis, and Computational Studies
title_full_unstemmed Novel Quinoline- and Naphthalene-Incorporated Hydrazineylidene–Propenamide Analogues as Antidiabetic Agents: Design, Synthesis, and Computational Studies
title_short Novel Quinoline- and Naphthalene-Incorporated Hydrazineylidene–Propenamide Analogues as Antidiabetic Agents: Design, Synthesis, and Computational Studies
title_sort novel quinoline and naphthalene incorporated hydrazineylidene propenamide analogues as antidiabetic agents design synthesis and computational studies
topic quinoline
naphthalene
α-glycosidase
α-amylase and aldose reductase enzyme inhibitions
molecular docking simulation
molecular dynamics simulation
url https://www.mdpi.com/1424-8247/17/12/1692
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