Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV)
During the present study, an endeavor has been made to produce a simple, rapid, and simultaneous method for determination of phenolic compounds by using high-performance liquid chromatography in aerial parts of Leptadenia pyrotechnica (Forssk.) Decne. collected from the Indian Thar Desert. The optim...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Advances in Pharmacological Sciences |
| Online Access: | http://dx.doi.org/10.1155/2018/9604972 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832565544030568448 |
|---|---|
| author | Raman Preet Raghbir Chand Gupta |
| author_facet | Raman Preet Raghbir Chand Gupta |
| author_sort | Raman Preet |
| collection | DOAJ |
| description | During the present study, an endeavor has been made to produce a simple, rapid, and simultaneous method for determination of phenolic compounds by using high-performance liquid chromatography in aerial parts of Leptadenia pyrotechnica (Forssk.) Decne. collected from the Indian Thar Desert. The optimized process was used for the quantification of ten phenolic compounds. The chromatographic separation was accomplished on an Atlantis T3 column at 25°C with isocratic elution. A mixture of acetonitrile and water was used as the mobile phase at a flow rate of 0.8 mL/min. The linear regression examination data for the calibration plots displayed a good linear relationship with r2 > 0.999 in the concentration range of 2–20 µL. In the methanolic extracts of the whole plant of L. pyrotechnica, the content of caffeic acid (3.3%) was reported to be the highest concentration. |
| format | Article |
| id | doaj-art-e183e8b17fd04aada85be2d66054c2a4 |
| institution | Kabale University |
| issn | 1687-6334 1687-6342 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological Sciences |
| spelling | doaj-art-e183e8b17fd04aada85be2d66054c2a42025-02-03T01:07:21ZengWileyAdvances in Pharmacological Sciences1687-63341687-63422018-01-01201810.1155/2018/96049729604972Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV)Raman Preet0Raghbir Chand Gupta1Department of Botany, Punjabi University, Patiala 147002, Punjab, IndiaDepartment of Botany, Punjabi University, Patiala 147002, Punjab, IndiaDuring the present study, an endeavor has been made to produce a simple, rapid, and simultaneous method for determination of phenolic compounds by using high-performance liquid chromatography in aerial parts of Leptadenia pyrotechnica (Forssk.) Decne. collected from the Indian Thar Desert. The optimized process was used for the quantification of ten phenolic compounds. The chromatographic separation was accomplished on an Atlantis T3 column at 25°C with isocratic elution. A mixture of acetonitrile and water was used as the mobile phase at a flow rate of 0.8 mL/min. The linear regression examination data for the calibration plots displayed a good linear relationship with r2 > 0.999 in the concentration range of 2–20 µL. In the methanolic extracts of the whole plant of L. pyrotechnica, the content of caffeic acid (3.3%) was reported to be the highest concentration.http://dx.doi.org/10.1155/2018/9604972 |
| spellingShingle | Raman Preet Raghbir Chand Gupta Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV) Advances in Pharmacological Sciences |
| title | Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV) |
| title_full | Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV) |
| title_fullStr | Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV) |
| title_full_unstemmed | Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV) |
| title_short | Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV) |
| title_sort | simultaneous determination of phenolic compounds in leptadenia pyrotechnica forssk decne by using high performance liquid chromatography hplc dad uv |
| url | http://dx.doi.org/10.1155/2018/9604972 |
| work_keys_str_mv | AT ramanpreet simultaneousdeterminationofphenoliccompoundsinleptadeniapyrotechnicaforsskdecnebyusinghighperformanceliquidchromatographyhplcdaduv AT raghbirchandgupta simultaneousdeterminationofphenoliccompoundsinleptadeniapyrotechnicaforsskdecnebyusinghighperformanceliquidchromatographyhplcdaduv |