Non-targeted LC-MS metabolomics reveal shifts from wound-induced enzymatic browning to lignification during extended storage of fresh-cut lettuce in modified atmosphere packaging
Modified Atmosphere Packaging (MAP) is a conventional method used to prolong the shelf-life of fresh-cut vegetables, including lettuce. However, MAP-stored lettuce remains perishable, and its deterioration mechanism is not fully understood. Here, we utilized non-targeted LC-MS metabolomics to evalua...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Current Research in Food Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2665927124002867 |
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| author | Fanny Widjaja Priscille Steensma Leevi Annala Arto Klami Saijaliisa Kangasjärvi Mari Lehtonen Kirsi S. Mikkonen |
| author_facet | Fanny Widjaja Priscille Steensma Leevi Annala Arto Klami Saijaliisa Kangasjärvi Mari Lehtonen Kirsi S. Mikkonen |
| author_sort | Fanny Widjaja |
| collection | DOAJ |
| description | Modified Atmosphere Packaging (MAP) is a conventional method used to prolong the shelf-life of fresh-cut vegetables, including lettuce. However, MAP-stored lettuce remains perishable, and its deterioration mechanism is not fully understood. Here, we utilized non-targeted LC-MS metabolomics to evaluate the effects of cutting and extended storage time on metabolite profiles of lettuce stored in MAP. Additionally, hyperspectral imaging was used to measure perceptual changes. Our findings reveal a bipartite response to wounding. In early storage, enzymatic browning was the main response to wounding, evidenced by accumulation of caffeic acid derivatives and flavonoids, substrates for polyphenol oxidases. As storage progressed, enzymatic browning was inhibited, and a shift towards lignification became apparent, evidenced by accumulation of monolignol derivatives. These findings offer new insights into the deterioration mechanism of fresh-cut lettuce occurring in MAP. |
| format | Article |
| id | doaj-art-e15064b57e0c41438dd427025008f72f |
| institution | OA Journals |
| issn | 2665-9271 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Food Science |
| spelling | doaj-art-e15064b57e0c41438dd427025008f72f2025-08-20T02:06:30ZengElsevierCurrent Research in Food Science2665-92712025-01-011010095910.1016/j.crfs.2024.100959Non-targeted LC-MS metabolomics reveal shifts from wound-induced enzymatic browning to lignification during extended storage of fresh-cut lettuce in modified atmosphere packagingFanny Widjaja0Priscille Steensma1Leevi Annala2Arto Klami3Saijaliisa Kangasjärvi4Mari Lehtonen5Kirsi S. Mikkonen6Department of Food and Nutrition, University of Helsinki, P.O. Box 66, 00014, Helsinki, Finland; Corresponding author.Department of Food and Nutrition, University of Helsinki, P.O. Box 66, 00014, Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, 00014, Helsinki, FinlandDepartment of Computer Science, University of Helsinki, P.O. Box 68, 00014, Helsinki, FinlandOrganismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, P.O. Box 65, 00014, Helsinki, Finland; Department of Agricultural Sciences, Faculty of Agriculture and Forestry, University of Helsinki, P.O. Box 65, 00014, Helsinki, Finland; Viikki Plant Science Center, University of Helsinki, P.O. Box 65, 00014, Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, 00014, Helsinki, FinlandDepartment of Food and Nutrition, University of Helsinki, P.O. Box 66, 00014, Helsinki, Finland; Helsinki Institute of Sustainability Science (HELSUS), University of Helsinki, P.O. Box 65, 00014, Helsinki, FinlandModified Atmosphere Packaging (MAP) is a conventional method used to prolong the shelf-life of fresh-cut vegetables, including lettuce. However, MAP-stored lettuce remains perishable, and its deterioration mechanism is not fully understood. Here, we utilized non-targeted LC-MS metabolomics to evaluate the effects of cutting and extended storage time on metabolite profiles of lettuce stored in MAP. Additionally, hyperspectral imaging was used to measure perceptual changes. Our findings reveal a bipartite response to wounding. In early storage, enzymatic browning was the main response to wounding, evidenced by accumulation of caffeic acid derivatives and flavonoids, substrates for polyphenol oxidases. As storage progressed, enzymatic browning was inhibited, and a shift towards lignification became apparent, evidenced by accumulation of monolignol derivatives. These findings offer new insights into the deterioration mechanism of fresh-cut lettuce occurring in MAP.http://www.sciencedirect.com/science/article/pii/S2665927124002867Fresh-cut vegetablesSenescenceQuality deteriorationPhenylpropanoid pathwayHyperspectral imaging |
| spellingShingle | Fanny Widjaja Priscille Steensma Leevi Annala Arto Klami Saijaliisa Kangasjärvi Mari Lehtonen Kirsi S. Mikkonen Non-targeted LC-MS metabolomics reveal shifts from wound-induced enzymatic browning to lignification during extended storage of fresh-cut lettuce in modified atmosphere packaging Current Research in Food Science Fresh-cut vegetables Senescence Quality deterioration Phenylpropanoid pathway Hyperspectral imaging |
| title | Non-targeted LC-MS metabolomics reveal shifts from wound-induced enzymatic browning to lignification during extended storage of fresh-cut lettuce in modified atmosphere packaging |
| title_full | Non-targeted LC-MS metabolomics reveal shifts from wound-induced enzymatic browning to lignification during extended storage of fresh-cut lettuce in modified atmosphere packaging |
| title_fullStr | Non-targeted LC-MS metabolomics reveal shifts from wound-induced enzymatic browning to lignification during extended storage of fresh-cut lettuce in modified atmosphere packaging |
| title_full_unstemmed | Non-targeted LC-MS metabolomics reveal shifts from wound-induced enzymatic browning to lignification during extended storage of fresh-cut lettuce in modified atmosphere packaging |
| title_short | Non-targeted LC-MS metabolomics reveal shifts from wound-induced enzymatic browning to lignification during extended storage of fresh-cut lettuce in modified atmosphere packaging |
| title_sort | non targeted lc ms metabolomics reveal shifts from wound induced enzymatic browning to lignification during extended storage of fresh cut lettuce in modified atmosphere packaging |
| topic | Fresh-cut vegetables Senescence Quality deterioration Phenylpropanoid pathway Hyperspectral imaging |
| url | http://www.sciencedirect.com/science/article/pii/S2665927124002867 |
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