Protocol for identifying components of subcellular compartments by antibody-based in situ biotinylation

Summary: Recent studies revealed that membrane-less subcellular organelles play important roles in cellular functions. Here, we present a protocol for identifying subcellular compartment components by antibody-based in situ biotinylation. We describe steps for in situ biotinylation labeling using a...

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Bibliographic Details
Main Authors: Hidefumi Suzuki, Kexin Li, Sayaka Ito, Keiichiro Asano, Keisuke Noguchi, Ryota Abe, Hidehisa Takahashi
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002096
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