Measuring the Thermal Unfolding of Lysozyme: A Critical Comparison of Differential Scanning Fluorimetry and Differential Scanning Calorimetry

Abstract The thermal unfolding of lysozyme in aqueous solution has been analyzed by (nano) differential scanning fluorimetry (nanoDSF) and differential scanning calorimetry (DSC). In addition, dynamic light scattering (DLS) acquired in parallel to the DSF measurements, was used to confirm that the c...

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Bibliographic Details
Main Authors: Weronika Malicka, Yueyue Dai, Andreas Herrmann, Rainer Haag, Matthias Ballauff, Marina Pigaleva, Thomas Risse, Daniel Lauster, Iman Asakereh, Mazdak Khajehpour
Format: Article
Language:English
Published: Wiley-VCH 2025-06-01
Series:ChemistryOpen
Online Access:https://doi.org/10.1002/open.202400340
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