Application of MALDI-TOF MS and FT-IR spectroscopy in identification and antibiotic resistance profiling of lactic acid bacteria

Application of MALDI-TOF MS and FT-IR spectroscopy in identification and antibiotic resistance profiling of lactic acid bacteria

Abstract This study evaluates the combined application of MALDI-TOF MS and Fourier transform infrared (FT-IR) spectroscopy for the purpose of species identification and strain-level differentiation of lactic acid bacteria isolated from commercial yoghurts. MALDI-TOF MS provided rapid species-level i...

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Bibliographic Details
Main Authors: Tamás Kocsis, Anna Győrffy, Andrea Pomázi
Format: Article
Language:English
Published: Springer 2025-07-01
Series:Applied Microbiology and Biotechnology
Subjects:
Antibiotic resistance
FT-IR Biotyper
Lactic acid bacteria
MALDI-TOF MS
Phenotypic profiling
Online Access:https://doi.org/10.1007/s00253-025-13550-x
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Summary:Abstract This study evaluates the combined application of MALDI-TOF MS and Fourier transform infrared (FT-IR) spectroscopy for the purpose of species identification and strain-level differentiation of lactic acid bacteria isolated from commercial yoghurts. MALDI-TOF MS provided rapid species-level identification, while FT-IR detected metabolic variations by analysing key cellular components such as membrane fatty acids (3000–2800 cm−1), protein amide bands (1800–1500 cm−1), and polysaccharides (1200–900 cm−1). These spectral features reflected phenotypic differences among strains linked to antibiotic resistance profiles. Disc diffusion antibiotic susceptibility testing revealed significant variability among isolates, and a strong correlation was observed between FT-IR-derived linear discriminant analysis (LDA) clusters and resistance to oxacillin, clindamycin, and tetracycline (Chi2 test, p < 0.05). This demonstrates FT-IR as a rapid, non-invasive tool for the early detection of resistant strains, facilitating real-time monitoring of bacterial adaptation during fermentation. The findings of this study provide support for integrating FT-IR and MALDI-TOF MS into industrial quality control, aiding in strain selection and enhancing food safety. Key points • FT-IR enables rapid phenotypic typing of lactic acid bacteria strains. • Spectral profiles correlate with antibiotic resistance. • MALDI-TOF MS and FT-IR offer a robust, complementary identification method.
ISSN:1432-0614

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