Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma
Abstract Background Treatments based on the inhibition of pivotal signals of cancer stem cells (CSCs) are on a promising track. Recent studies have shown that targeting CSCs with broader immune‐based therapeutic methods, for example, the anti‐CD47 treatment, may serve as a more potent strategy for e...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-03-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.1894 |
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| author | Jian‐Hua Wang Shu‐Ting Huang Lan Zhang Zi‐Gang Liu Rong‐Xin Liang Sen‐Wei Jiang Yi‐Nan Jiang Xing‐Juan Yu Yu‐Chuan Jiang Xi‐Zhao Li Pei‐Fen Zhang Zhe‐Sheng Wen Min Zheng |
| author_facet | Jian‐Hua Wang Shu‐Ting Huang Lan Zhang Zi‐Gang Liu Rong‐Xin Liang Sen‐Wei Jiang Yi‐Nan Jiang Xing‐Juan Yu Yu‐Chuan Jiang Xi‐Zhao Li Pei‐Fen Zhang Zhe‐Sheng Wen Min Zheng |
| author_sort | Jian‐Hua Wang |
| collection | DOAJ |
| description | Abstract Background Treatments based on the inhibition of pivotal signals of cancer stem cells (CSCs) are on a promising track. Recent studies have shown that targeting CSCs with broader immune‐based therapeutic methods, for example, the anti‐CD47 treatment, may serve as a more potent strategy for eliminating these intractable cells. We aimed to explore the prognostic effects of CD47/CD133 and the potential therapeutic significance of CD47 in esophageal squamous cell carcinoma (ESCC). Methods Immunohistochemistry was employed to identify the characteristics of CD47 and CD133 in 26 pairs of tumor tissues and adjacent non‐tumor tissues and 136 ESCC tissues. Kaplan‐Meier analysis and Cox proportional hazards models were built for estimating the prognostic values of CD47 and CD133 expression and their combined stemness index. Sphere formation assays were undertaken to explore the effects of CD47 inhibition on primary human ESCC CSCs. Results Results conclude that CD47 and CD133 expression is increased in tumor tissues as compared to adjacent non‐tumor tissues. A positive correlation between CD47/CD133 expression and differentiation was found in 136 ESCC patients. Survival analysis indicated that patients with high CD47 or CD133 expression exhibited poor overall survival and progression‐free survival (PFS). The combination of high CD47 and CD133 expression was a reliable independent prognostic factor for both OS (HR = 1.940, 95% CI = 1.399‐2.690, P < 0.0001) and progression‐free survival (HR = 1.883, 95% CI = 1.384‐2.562, P < 0.0001). Notably, CD47+ CD133+ ESCC cells were observed to possess the characteristics of CSCs, and anti‐CD47 treatment veritably eliminated the CSCs pool. Conclusions The stemness index determined by the expression of CD47 and CD133 is a promising prognostic predictor, and CD47 is a potential therapeutic target for CSCs in ESCC patients. |
| format | Article |
| id | doaj-art-de7bc1465c9749f2a3322bb9db9213cf |
| institution | Kabale University |
| issn | 2045-7634 |
| language | English |
| publishDate | 2019-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-de7bc1465c9749f2a3322bb9db9213cf2025-01-31T08:47:43ZengWileyCancer Medicine2045-76342019-03-01831315132510.1002/cam4.1894Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinomaJian‐Hua Wang0Shu‐Ting Huang1Lan Zhang2Zi‐Gang Liu3Rong‐Xin Liang4Sen‐Wei Jiang5Yi‐Nan Jiang6Xing‐Juan Yu7Yu‐Chuan Jiang8Xi‐Zhao Li9Pei‐Fen Zhang10Zhe‐Sheng Wen11Min Zheng12Department of Chest Second People’s Hospital of Guangdong Province Guangzhou ChinaDepartment of Gynecology Sun Yat‐Sen University Cancer Center Guangzhou ChinaDepartment of Gynecology Sun Yat‐Sen University Cancer Center Guangzhou ChinaDepartment of Chest Second People’s Hospital of Guangdong Province Guangzhou ChinaDepartment of Chest Second People’s Hospital of Guangdong Province Guangzhou ChinaDepartment of Gynecology Sun Yat‐Sen University Cancer Center Guangzhou ChinaDepartment of Gynecology Sun Yat‐Sen University Cancer Center Guangzhou ChinaState Key Laboratory of Oncology in South China Guangzhou ChinaDepartment of Chest Sun Yat‐Sen University Cancer Center Guangzhou ChinaState Key Laboratory of Oncology in South China Guangzhou ChinaState Key Laboratory of Oncology in South China Guangzhou ChinaDepartment of Chest Sun Yat‐Sen University Cancer Center Guangzhou ChinaDepartment of Gynecology Sun Yat‐Sen University Cancer Center Guangzhou ChinaAbstract Background Treatments based on the inhibition of pivotal signals of cancer stem cells (CSCs) are on a promising track. Recent studies have shown that targeting CSCs with broader immune‐based therapeutic methods, for example, the anti‐CD47 treatment, may serve as a more potent strategy for eliminating these intractable cells. We aimed to explore the prognostic effects of CD47/CD133 and the potential therapeutic significance of CD47 in esophageal squamous cell carcinoma (ESCC). Methods Immunohistochemistry was employed to identify the characteristics of CD47 and CD133 in 26 pairs of tumor tissues and adjacent non‐tumor tissues and 136 ESCC tissues. Kaplan‐Meier analysis and Cox proportional hazards models were built for estimating the prognostic values of CD47 and CD133 expression and their combined stemness index. Sphere formation assays were undertaken to explore the effects of CD47 inhibition on primary human ESCC CSCs. Results Results conclude that CD47 and CD133 expression is increased in tumor tissues as compared to adjacent non‐tumor tissues. A positive correlation between CD47/CD133 expression and differentiation was found in 136 ESCC patients. Survival analysis indicated that patients with high CD47 or CD133 expression exhibited poor overall survival and progression‐free survival (PFS). The combination of high CD47 and CD133 expression was a reliable independent prognostic factor for both OS (HR = 1.940, 95% CI = 1.399‐2.690, P < 0.0001) and progression‐free survival (HR = 1.883, 95% CI = 1.384‐2.562, P < 0.0001). Notably, CD47+ CD133+ ESCC cells were observed to possess the characteristics of CSCs, and anti‐CD47 treatment veritably eliminated the CSCs pool. Conclusions The stemness index determined by the expression of CD47 and CD133 is a promising prognostic predictor, and CD47 is a potential therapeutic target for CSCs in ESCC patients.https://doi.org/10.1002/cam4.1894CD133CD47CSCsESCCprognosistherapeutic |
| spellingShingle | Jian‐Hua Wang Shu‐Ting Huang Lan Zhang Zi‐Gang Liu Rong‐Xin Liang Sen‐Wei Jiang Yi‐Nan Jiang Xing‐Juan Yu Yu‐Chuan Jiang Xi‐Zhao Li Pei‐Fen Zhang Zhe‐Sheng Wen Min Zheng Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma Cancer Medicine CD133 CD47 CSCs ESCC prognosis therapeutic |
| title | Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma |
| title_full | Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma |
| title_fullStr | Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma |
| title_full_unstemmed | Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma |
| title_short | Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma |
| title_sort | combined prognostic value of the cancer stem cell markers cd47 and cd133 in esophageal squamous cell carcinoma |
| topic | CD133 CD47 CSCs ESCC prognosis therapeutic |
| url | https://doi.org/10.1002/cam4.1894 |
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