High soybean dietary supplementation with quercetin improves antioxidant capacity of spotted sea bass Lateolabrax maculatus

This study aimed to examine the effect of quercetin (QUE) on the antioxidant capacity of spotted sea bass that were fed a high soybean meal diet. 44 % fish meal was used as a healthy control, and soybean meal instead of 50 % fish meal induced a negative reaction. On this basis, 0.25, 0.50, 0.75, and...

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Main Authors: Longhui Liu, Yanbo Zhao, Zhangfan Huang, Zhongying Long, Huihui Qin, Hao Lin, Sishun Zhou, Lumin Kong, Jianrong Ma, Yi Lin, Zhongbao Li
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Aquaculture Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352513424005179
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author Longhui Liu
Yanbo Zhao
Zhangfan Huang
Zhongying Long
Huihui Qin
Hao Lin
Sishun Zhou
Lumin Kong
Jianrong Ma
Yi Lin
Zhongbao Li
author_facet Longhui Liu
Yanbo Zhao
Zhangfan Huang
Zhongying Long
Huihui Qin
Hao Lin
Sishun Zhou
Lumin Kong
Jianrong Ma
Yi Lin
Zhongbao Li
author_sort Longhui Liu
collection DOAJ
description This study aimed to examine the effect of quercetin (QUE) on the antioxidant capacity of spotted sea bass that were fed a high soybean meal diet. 44 % fish meal was used as a healthy control, and soybean meal instead of 50 % fish meal induced a negative reaction. On this basis, 0.25, 0.50, 0.75, and 1.00 g/kg QUE were added, respectively. A total of 540 spotted sea bass were randomly allocated into 6 groups and provided with diets that corresponded to their respective groups for a duration of 56 days. The results showed a significant decrease in the antioxidant capacity of spotted sea bass fed a high soybean meal diet. The inclusion of QUE considerably improved the antioxidant capacity of spotted sea bass. This was evidenced by the notable rise in glutathione (GSH) levels in the intestine and liver, as well as the elevated levels of catalase (CAT) and total antioxidant capacity (T-AOC) in the liver. Furthermore, transcriptome results showed that the S-transferase (GST) gene in the glutathione metabolism pathway was significantly down-regulated by high soybean meal, while with the addition of QUE, noteworthy alterations in genes associated with the PI3K-Akt signaling pathway, Foxo signaling pathway, and AMPK signaling pathway. These signaling pathways are involved in signal transduction, apoptosis, and oxidative stress. On the other hand, genes related to energy metabolism, such as phosphoenolpyruvate carboxykinase (PEPCK), were observed to be upregulated. These pathways could potentially contribute to the antioxidant properties of QUE.
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publishDate 2024-12-01
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series Aquaculture Reports
spelling doaj-art-de270c72f63e4371a4a4af4e2f726a612025-08-20T02:51:30ZengElsevierAquaculture Reports2352-51342024-12-013910242910.1016/j.aqrep.2024.102429High soybean dietary supplementation with quercetin improves antioxidant capacity of spotted sea bass Lateolabrax maculatusLonghui Liu0Yanbo Zhao1Zhangfan Huang2Zhongying Long3Huihui Qin4Hao Lin5Sishun Zhou6Lumin Kong7Jianrong Ma8Yi Lin9Zhongbao Li10Fisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, ChinaFisheries College, Jimei University, Xiamen, China; Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen, China; Correspondence to: Fisheries College, Jimei University, Xiamen, Fujian, China.This study aimed to examine the effect of quercetin (QUE) on the antioxidant capacity of spotted sea bass that were fed a high soybean meal diet. 44 % fish meal was used as a healthy control, and soybean meal instead of 50 % fish meal induced a negative reaction. On this basis, 0.25, 0.50, 0.75, and 1.00 g/kg QUE were added, respectively. A total of 540 spotted sea bass were randomly allocated into 6 groups and provided with diets that corresponded to their respective groups for a duration of 56 days. The results showed a significant decrease in the antioxidant capacity of spotted sea bass fed a high soybean meal diet. The inclusion of QUE considerably improved the antioxidant capacity of spotted sea bass. This was evidenced by the notable rise in glutathione (GSH) levels in the intestine and liver, as well as the elevated levels of catalase (CAT) and total antioxidant capacity (T-AOC) in the liver. Furthermore, transcriptome results showed that the S-transferase (GST) gene in the glutathione metabolism pathway was significantly down-regulated by high soybean meal, while with the addition of QUE, noteworthy alterations in genes associated with the PI3K-Akt signaling pathway, Foxo signaling pathway, and AMPK signaling pathway. These signaling pathways are involved in signal transduction, apoptosis, and oxidative stress. On the other hand, genes related to energy metabolism, such as phosphoenolpyruvate carboxykinase (PEPCK), were observed to be upregulated. These pathways could potentially contribute to the antioxidant properties of QUE.http://www.sciencedirect.com/science/article/pii/S2352513424005179QuercetinSpotted sea bassHigh soybean meal dietAntioxidantTranscriptomics
spellingShingle Longhui Liu
Yanbo Zhao
Zhangfan Huang
Zhongying Long
Huihui Qin
Hao Lin
Sishun Zhou
Lumin Kong
Jianrong Ma
Yi Lin
Zhongbao Li
High soybean dietary supplementation with quercetin improves antioxidant capacity of spotted sea bass Lateolabrax maculatus
Aquaculture Reports
Quercetin
Spotted sea bass
High soybean meal diet
Antioxidant
Transcriptomics
title High soybean dietary supplementation with quercetin improves antioxidant capacity of spotted sea bass Lateolabrax maculatus
title_full High soybean dietary supplementation with quercetin improves antioxidant capacity of spotted sea bass Lateolabrax maculatus
title_fullStr High soybean dietary supplementation with quercetin improves antioxidant capacity of spotted sea bass Lateolabrax maculatus
title_full_unstemmed High soybean dietary supplementation with quercetin improves antioxidant capacity of spotted sea bass Lateolabrax maculatus
title_short High soybean dietary supplementation with quercetin improves antioxidant capacity of spotted sea bass Lateolabrax maculatus
title_sort high soybean dietary supplementation with quercetin improves antioxidant capacity of spotted sea bass lateolabrax maculatus
topic Quercetin
Spotted sea bass
High soybean meal diet
Antioxidant
Transcriptomics
url http://www.sciencedirect.com/science/article/pii/S2352513424005179
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