A γ-cyclodextrin emulsion loaded β-carotene and egg white peptides: construction, characterization, and application

In this study, we developed a kind of γ-cyclodextrin (γ-CD) emulsion that could encapsulate β-carotene and egg white peptides with high encapsulation efficiency. Commonly used indicators of physical and chemical properties such as encapsulation efficiency, loading rate, and droplet size were used to...

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Main Authors: Jingbo Liu, Zihao Li, Menghan Fu, Shengrao Li, Siwen Lü, Ting Zhang
Format: Article
Language:English
Published: Tsinghua University Press 2025-06-01
Series:Food Science of Animal Products
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Online Access:https://www.sciopen.com/article/10.26599/FSAP.2025.9240117
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author Jingbo Liu
Zihao Li
Menghan Fu
Shengrao Li
Siwen Lü
Ting Zhang
author_facet Jingbo Liu
Zihao Li
Menghan Fu
Shengrao Li
Siwen Lü
Ting Zhang
author_sort Jingbo Liu
collection DOAJ
description In this study, we developed a kind of γ-cyclodextrin (γ-CD) emulsion that could encapsulate β-carotene and egg white peptides with high encapsulation efficiency. Commonly used indicators of physical and chemical properties such as encapsulation efficiency, loading rate, and droplet size were used to evaluate the emulsion. Furthermore, we confirmed the binding condition through molecular forces and Fourier transform infrared (FTIR) spectroscopy. After that, we clarified that the binding mode between γ-CD and β-carotene was dominated by hydrophobic interaction forces, and further detected that the binding mode of γ-CD with egg white peptides was dominated by hydrogen bonding forces. The two emulsions were characterized by rheological properties, changes in environmental factors (temperature, ions, pH, and freeze-thaw), and it was clarified that the two Pickering emulsions were stable at different temperatures (up to 80 °C), ionic strengths (0−0.25 mol/L), weakly acidic and alkaline conditions, and the number of freeze-thawing times of two or less. In addition, based on the study of egg white peptide-loaded emulsions, egg white peptide-added coconut milk products were prepared and investigated, and it was demonstrated that the properties of the products were improved by the addition of bioactive peptide emulsions. Our study provided a theoretical basis for the application of egg white peptides and broadened the development of bioactive peptides in beverage products.
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publishDate 2025-06-01
publisher Tsinghua University Press
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series Food Science of Animal Products
spelling doaj-art-dde817a775e641fa95a7c1f0e06f44762025-08-20T03:05:42ZengTsinghua University PressFood Science of Animal Products2958-41242958-37802025-06-0132924011710.26599/FSAP.2025.9240117A γ-cyclodextrin emulsion loaded β-carotene and egg white peptides: construction, characterization, and applicationJingbo Liu0Zihao Li1Menghan Fu2Shengrao Li3Siwen Lü4Ting Zhang5Jilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, ChinaJilin Provincial Key Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, ChinaIn this study, we developed a kind of γ-cyclodextrin (γ-CD) emulsion that could encapsulate β-carotene and egg white peptides with high encapsulation efficiency. Commonly used indicators of physical and chemical properties such as encapsulation efficiency, loading rate, and droplet size were used to evaluate the emulsion. Furthermore, we confirmed the binding condition through molecular forces and Fourier transform infrared (FTIR) spectroscopy. After that, we clarified that the binding mode between γ-CD and β-carotene was dominated by hydrophobic interaction forces, and further detected that the binding mode of γ-CD with egg white peptides was dominated by hydrogen bonding forces. The two emulsions were characterized by rheological properties, changes in environmental factors (temperature, ions, pH, and freeze-thaw), and it was clarified that the two Pickering emulsions were stable at different temperatures (up to 80 °C), ionic strengths (0−0.25 mol/L), weakly acidic and alkaline conditions, and the number of freeze-thawing times of two or less. In addition, based on the study of egg white peptide-loaded emulsions, egg white peptide-added coconut milk products were prepared and investigated, and it was demonstrated that the properties of the products were improved by the addition of bioactive peptide emulsions. Our study provided a theoretical basis for the application of egg white peptides and broadened the development of bioactive peptides in beverage products.https://www.sciopen.com/article/10.26599/FSAP.2025.9240117γ-cyclodextrinegg white peptidespickering emulsionemulsion stabilitymilk beverage products
spellingShingle Jingbo Liu
Zihao Li
Menghan Fu
Shengrao Li
Siwen Lü
Ting Zhang
A γ-cyclodextrin emulsion loaded β-carotene and egg white peptides: construction, characterization, and application
Food Science of Animal Products
γ-cyclodextrin
egg white peptides
pickering emulsion
emulsion stability
milk beverage products
title A γ-cyclodextrin emulsion loaded β-carotene and egg white peptides: construction, characterization, and application
title_full A γ-cyclodextrin emulsion loaded β-carotene and egg white peptides: construction, characterization, and application
title_fullStr A γ-cyclodextrin emulsion loaded β-carotene and egg white peptides: construction, characterization, and application
title_full_unstemmed A γ-cyclodextrin emulsion loaded β-carotene and egg white peptides: construction, characterization, and application
title_short A γ-cyclodextrin emulsion loaded β-carotene and egg white peptides: construction, characterization, and application
title_sort γ cyclodextrin emulsion loaded β carotene and egg white peptides construction characterization and application
topic γ-cyclodextrin
egg white peptides
pickering emulsion
emulsion stability
milk beverage products
url https://www.sciopen.com/article/10.26599/FSAP.2025.9240117
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