Transfer of Cytoplasmic Male Sterility to the Female Parents of Heat- and Drought-Resilient Maize (<i>Zea mays</i> L.) Hybrids
Maize is the second most important staple food crop in the world after wheat. For maize hybrid seed production, a prominent step is detasseling in the female parent, which is laborious, time consuming, and increases the hybrid seed cost by 15 to 20 percent. Hence, to overcome this problem, exploitat...
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2025-01-01
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| author | Ayyanagouda Patil Kushal Gowda Shivananda T. Lakshman Prakash H. Kuchanur Gajanan Saykhedkar Sudha Krishnan Nair Kisan B. Jadhav Sharanabasappa Yeri Gururaj Sunkad Jayaprakash M. Nidagundi Vinayan Madhumal Thayil Pervez H. Zaidi |
| author_facet | Ayyanagouda Patil Kushal Gowda Shivananda T. Lakshman Prakash H. Kuchanur Gajanan Saykhedkar Sudha Krishnan Nair Kisan B. Jadhav Sharanabasappa Yeri Gururaj Sunkad Jayaprakash M. Nidagundi Vinayan Madhumal Thayil Pervez H. Zaidi |
| author_sort | Ayyanagouda Patil |
| collection | DOAJ |
| description | Maize is the second most important staple food crop in the world after wheat. For maize hybrid seed production, a prominent step is detasseling in the female parent, which is laborious, time consuming, and increases the hybrid seed cost by 15 to 20 percent. Hence, to overcome this problem, exploitation of male sterility in maize crops gains special significance. In this direction, the research was conducted to transfer cytoplasmic male sterility (CMS-C) from a CMS donor (VL192114) into the female parents (CAL1514 and ZL153493) of heat- and drought-resilient maize hybrids (RCRMH-2 and RCRMH-3) by a marker-assisted backcross scheme. The present research used Diversity Array Technology (DArTag) and Kompetitive Allele Specific PCR (KASP)-based single-nucleotide polymorphic markers for background selection in backcross populations. Genome recovery percentage ranged from 64.25 to 72.70, 78.94 to 87.69 and 82.28 to 90.77 percent in the BC<sub>1</sub>F<sub>1</sub>, BC<sub>2</sub>F<sub>1</sub> and BC<sub>3</sub>F<sub>1</sub> population, respectively, in the CAL1514 population, while it was 63.47 to 73.55, 78.16 to 88.76 and 83.96 to 91.81 percent in the BC<sub>1</sub>F<sub>1</sub>, BC<sub>2</sub>F<sub>1</sub> and BC<sub>3</sub>F<sub>1</sub> population, respectively, in the ZL153493 population. When the near-isogenic CMS lines of both populations are compared for agro-morphological traits with their recurrent parents, the agronomic qualities of recurrent parents, as well as the attributes of distinctness, uniformity and stability, are shown. Therefore, male sterility-transferred, female lines of RCRMH-2 and RCRMH-3 maize hybrids can be used directly to produce maize hybrid seed without the need of the detasseling process. |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-dd6a7942fea048af965285331f3b1fa72025-01-24T13:16:43ZengMDPI AGAgronomy2073-43952025-01-011519810.3390/agronomy15010098Transfer of Cytoplasmic Male Sterility to the Female Parents of Heat- and Drought-Resilient Maize (<i>Zea mays</i> L.) HybridsAyyanagouda Patil0Kushal Gowda1Shivananda T. Lakshman2Prakash H. Kuchanur3Gajanan Saykhedkar4Sudha Krishnan Nair5Kisan B. Jadhav6Sharanabasappa Yeri7Gururaj Sunkad8Jayaprakash M. Nidagundi9Vinayan Madhumal Thayil10Pervez H. Zaidi11Department of Molecular Biology and Agricultural Biotechnology, University of Agricultural Sciences, Raichur 584104, Karnataka, IndiaDepartment of Molecular Biology and Agricultural Biotechnology, University of Agricultural Sciences, Raichur 584104, Karnataka, IndiaDepartment of Molecular Biology and Agricultural Biotechnology, University of Agricultural Sciences, Raichur 584104, Karnataka, IndiaDepartment of Molecular Biology and Agricultural Biotechnology, University of Agricultural Sciences, Raichur 584104, Karnataka, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), CIMMYT-Asia, Patancheru 502324, Telangana, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), CIMMYT-Asia, Patancheru 502324, Telangana, IndiaDepartment of Molecular Biology and Agricultural Biotechnology, University of Agricultural Sciences, Raichur 584104, Karnataka, IndiaDepartment of Molecular Biology and Agricultural Biotechnology, University of Agricultural Sciences, Raichur 584104, Karnataka, IndiaDepartment of Molecular Biology and Agricultural Biotechnology, University of Agricultural Sciences, Raichur 584104, Karnataka, IndiaDepartment of Molecular Biology and Agricultural Biotechnology, University of Agricultural Sciences, Raichur 584104, Karnataka, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), CIMMYT-Asia, Patancheru 502324, Telangana, IndiaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), CIMMYT-Asia, Patancheru 502324, Telangana, IndiaMaize is the second most important staple food crop in the world after wheat. For maize hybrid seed production, a prominent step is detasseling in the female parent, which is laborious, time consuming, and increases the hybrid seed cost by 15 to 20 percent. Hence, to overcome this problem, exploitation of male sterility in maize crops gains special significance. In this direction, the research was conducted to transfer cytoplasmic male sterility (CMS-C) from a CMS donor (VL192114) into the female parents (CAL1514 and ZL153493) of heat- and drought-resilient maize hybrids (RCRMH-2 and RCRMH-3) by a marker-assisted backcross scheme. The present research used Diversity Array Technology (DArTag) and Kompetitive Allele Specific PCR (KASP)-based single-nucleotide polymorphic markers for background selection in backcross populations. Genome recovery percentage ranged from 64.25 to 72.70, 78.94 to 87.69 and 82.28 to 90.77 percent in the BC<sub>1</sub>F<sub>1</sub>, BC<sub>2</sub>F<sub>1</sub> and BC<sub>3</sub>F<sub>1</sub> population, respectively, in the CAL1514 population, while it was 63.47 to 73.55, 78.16 to 88.76 and 83.96 to 91.81 percent in the BC<sub>1</sub>F<sub>1</sub>, BC<sub>2</sub>F<sub>1</sub> and BC<sub>3</sub>F<sub>1</sub> population, respectively, in the ZL153493 population. When the near-isogenic CMS lines of both populations are compared for agro-morphological traits with their recurrent parents, the agronomic qualities of recurrent parents, as well as the attributes of distinctness, uniformity and stability, are shown. Therefore, male sterility-transferred, female lines of RCRMH-2 and RCRMH-3 maize hybrids can be used directly to produce maize hybrid seed without the need of the detasseling process.https://www.mdpi.com/2073-4395/15/1/98cytoplasmic male sterilitydetasselingmarker-assisted backcrossSNP genotyping |
| spellingShingle | Ayyanagouda Patil Kushal Gowda Shivananda T. Lakshman Prakash H. Kuchanur Gajanan Saykhedkar Sudha Krishnan Nair Kisan B. Jadhav Sharanabasappa Yeri Gururaj Sunkad Jayaprakash M. Nidagundi Vinayan Madhumal Thayil Pervez H. Zaidi Transfer of Cytoplasmic Male Sterility to the Female Parents of Heat- and Drought-Resilient Maize (<i>Zea mays</i> L.) Hybrids Agronomy cytoplasmic male sterility detasseling marker-assisted backcross SNP genotyping |
| title | Transfer of Cytoplasmic Male Sterility to the Female Parents of Heat- and Drought-Resilient Maize (<i>Zea mays</i> L.) Hybrids |
| title_full | Transfer of Cytoplasmic Male Sterility to the Female Parents of Heat- and Drought-Resilient Maize (<i>Zea mays</i> L.) Hybrids |
| title_fullStr | Transfer of Cytoplasmic Male Sterility to the Female Parents of Heat- and Drought-Resilient Maize (<i>Zea mays</i> L.) Hybrids |
| title_full_unstemmed | Transfer of Cytoplasmic Male Sterility to the Female Parents of Heat- and Drought-Resilient Maize (<i>Zea mays</i> L.) Hybrids |
| title_short | Transfer of Cytoplasmic Male Sterility to the Female Parents of Heat- and Drought-Resilient Maize (<i>Zea mays</i> L.) Hybrids |
| title_sort | transfer of cytoplasmic male sterility to the female parents of heat and drought resilient maize i zea mays i l hybrids |
| topic | cytoplasmic male sterility detasseling marker-assisted backcross SNP genotyping |
| url | https://www.mdpi.com/2073-4395/15/1/98 |
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