Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis

Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis

Background The aim of this article is to evaluate the effect of different portions of Platelet Rich Fibrin (PRF) membranes and liquid-PRF, prepared by two distinct protocols/centrifuges each, on the multispecies subgingival biofilm.Materials and methods PRF membranes and liquid-PRF were prepared usi...

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Main Authors: Fabio Hideaki Uyeda, Gustavo Quilles Vargas, Larissa Matias Malavazi, Tatiane Tiemi Macedo, Aline Paim de Abreu Paulo Gomes, Manuela Rocha Bueno, Pedro Henrique Moreira Paulo Tolentino, Lucas Daylor Aguiar da Silva, Luciene Cristina Figueiredo, Jamil Awad Shibli, Bruno Bueno-Silva
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Journal of Oral Microbiology
Subjects:
Subgingival biofilm
periodontitis
antimicrobial
platelet-rich fibrin
iPRF
Online Access:https://www.tandfonline.com/doi/10.1080/20002297.2024.2445598
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Summary:Background The aim of this article is to evaluate the effect of different portions of Platelet Rich Fibrin (PRF) membranes and liquid-PRF, prepared by two distinct protocols/centrifuges each, on the multispecies subgingival biofilm.Materials and methods PRF membranes and liquid-PRF were prepared using two protocols: centrifuge 1 uses fixed acceleration while centrifuge 2, progressive acceleration. PRF samples were introduced into device concurrently with 33-species bacterial inoculum. After seven days, biofilm metabolic activity (MA) and microbial profile were evaluated through colorimetric reaction and DNA–DNA hybridization, respectively.Results Among PRF membranes, the ones from centrifuge 1 led to better reduction in MA, total biofilm, and F. periodonticum, P. gingivalis and T. forsythia counts when compared to untreated/centrifuge 2 treated biofilms. However, centrifuge 2 liquid-PRF reduced MA, total biofilm and F. periodonticum counts when compared to untreated/centrifuge 1 treated-biofilms.Conclusion PRF membrane and exhibited comparable antibiofilm activity. However, PRF distinct forms, obtained by same centrifugation protocol, may present different antimicrobial properties.
ISSN:2000-2297

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