Synergistic potential of agrobiomass-derived xylooligosaccharides (XOS) and antioxidants as pioneering prebiotics for probiotic cultivation

Prebiotic resources, such as xylooligosaccharides (XOS), which are resistant to acidity and temperature, can be derived from lignocellulosic agrobiomass. Hydrolysates containing prebiotic XOS were produced from fruit, rice, and sugarcane biomass using acid and hydrothermal pretreatments. Phosphoric...

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Main Authors: Nipaporn Chadathong, Surasak Siripornadulsil, Wilailak Siripornadulsil
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:Future Foods
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666833525001662
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author Nipaporn Chadathong
Surasak Siripornadulsil
Wilailak Siripornadulsil
author_facet Nipaporn Chadathong
Surasak Siripornadulsil
Wilailak Siripornadulsil
author_sort Nipaporn Chadathong
collection DOAJ
description Prebiotic resources, such as xylooligosaccharides (XOS), which are resistant to acidity and temperature, can be derived from lignocellulosic agrobiomass. Hydrolysates containing prebiotic XOS were produced from fruit, rice, and sugarcane biomass using acid and hydrothermal pretreatments. Phosphoric acid pretreatment showed greater potential for biomass breakdown and oligosaccharide release. FTIR analysis detected xylan and pectin in the pellets produced by precipitating the hydrolysates of mango peel (MP), pineapple peel (PP), rice bran (RB) and sugarcane leaf (SCL). The hydrolysate and the precipitate presented different XOS (xylobiose and xylotetraose) profiles and were more than twofold greater in the PP, RB, and SCL pellets (71.28, 109.55, and 188.48 mg/mL, respectively) than in the MP pellets (0.29 mg/mL). SCL hydrolysate, as a carbon source, promotes probiotic growth but is unsuitable for pathogen growth. Furthermore, fermenting the spray-dried SCL hydrolysate powder with probiotics (Bacillus subtilis and lactic acid bacteria) significantly increased its phenolic (236.07 µg GAE/mL) and flavonoid (2.75 mg QE/mL) contents and antioxidant activity (75.77 %). This study highlights the potential for a synergistic interaction between XOS and bioactive compounds, which may considerably benefit probiotics and their hosts. This research demonstrates an efficient and straightforward method for producing XOS, yielding prebiotics at 189.72 g/kg of biomass. This approach provides a viable alternative for the development of plant-based, value-added food products.
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spelling doaj-art-dce6f5d60293493a855c712af114eeb82025-08-20T03:34:00ZengElsevierFuture Foods2666-83352025-12-011210070710.1016/j.fufo.2025.100707Synergistic potential of agrobiomass-derived xylooligosaccharides (XOS) and antioxidants as pioneering prebiotics for probiotic cultivationNipaporn Chadathong0Surasak Siripornadulsil1Wilailak Siripornadulsil2Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, ThailandDepartment of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, ThailandCorresponding author.; Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, ThailandPrebiotic resources, such as xylooligosaccharides (XOS), which are resistant to acidity and temperature, can be derived from lignocellulosic agrobiomass. Hydrolysates containing prebiotic XOS were produced from fruit, rice, and sugarcane biomass using acid and hydrothermal pretreatments. Phosphoric acid pretreatment showed greater potential for biomass breakdown and oligosaccharide release. FTIR analysis detected xylan and pectin in the pellets produced by precipitating the hydrolysates of mango peel (MP), pineapple peel (PP), rice bran (RB) and sugarcane leaf (SCL). The hydrolysate and the precipitate presented different XOS (xylobiose and xylotetraose) profiles and were more than twofold greater in the PP, RB, and SCL pellets (71.28, 109.55, and 188.48 mg/mL, respectively) than in the MP pellets (0.29 mg/mL). SCL hydrolysate, as a carbon source, promotes probiotic growth but is unsuitable for pathogen growth. Furthermore, fermenting the spray-dried SCL hydrolysate powder with probiotics (Bacillus subtilis and lactic acid bacteria) significantly increased its phenolic (236.07 µg GAE/mL) and flavonoid (2.75 mg QE/mL) contents and antioxidant activity (75.77 %). This study highlights the potential for a synergistic interaction between XOS and bioactive compounds, which may considerably benefit probiotics and their hosts. This research demonstrates an efficient and straightforward method for producing XOS, yielding prebiotics at 189.72 g/kg of biomass. This approach provides a viable alternative for the development of plant-based, value-added food products.http://www.sciencedirect.com/science/article/pii/S2666833525001662AgrobiomassHydrolysatePrebioticsProbioticsSpray-driedXylooligosaccharide
spellingShingle Nipaporn Chadathong
Surasak Siripornadulsil
Wilailak Siripornadulsil
Synergistic potential of agrobiomass-derived xylooligosaccharides (XOS) and antioxidants as pioneering prebiotics for probiotic cultivation
Future Foods
Agrobiomass
Hydrolysate
Prebiotics
Probiotics
Spray-dried
Xylooligosaccharide
title Synergistic potential of agrobiomass-derived xylooligosaccharides (XOS) and antioxidants as pioneering prebiotics for probiotic cultivation
title_full Synergistic potential of agrobiomass-derived xylooligosaccharides (XOS) and antioxidants as pioneering prebiotics for probiotic cultivation
title_fullStr Synergistic potential of agrobiomass-derived xylooligosaccharides (XOS) and antioxidants as pioneering prebiotics for probiotic cultivation
title_full_unstemmed Synergistic potential of agrobiomass-derived xylooligosaccharides (XOS) and antioxidants as pioneering prebiotics for probiotic cultivation
title_short Synergistic potential of agrobiomass-derived xylooligosaccharides (XOS) and antioxidants as pioneering prebiotics for probiotic cultivation
title_sort synergistic potential of agrobiomass derived xylooligosaccharides xos and antioxidants as pioneering prebiotics for probiotic cultivation
topic Agrobiomass
Hydrolysate
Prebiotics
Probiotics
Spray-dried
Xylooligosaccharide
url http://www.sciencedirect.com/science/article/pii/S2666833525001662
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AT wilailaksiripornadulsil synergisticpotentialofagrobiomassderivedxylooligosaccharidesxosandantioxidantsaspioneeringprebioticsforprobioticcultivation