Pre-formation loading of extracellular vesicles with exogenous molecules using photoporation

Pre-formation loading of extracellular vesicles with exogenous molecules using photoporation

Abstract Despite the natural capacity of extracellular vesicles (EVs) to encapsulate intracellular compounds and transfer these to nearby or distant recipient cells, the intentional loading of EVs with cargo molecules remains a challenging endeavor. Pre-formation EV loading (i.e., during EV biogenes...

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Main Authors: Jana Ramon, Cláudio Pinheiro, Charysse Vandendriessche, Estefanía Lozano-Andrés, Herlinde De Keersmaecker, Deep Punj, Juan C. Fraire, Edward Geeurickx, Marca H. M. Wauben, Pieter Vader, Roosmarijn E. Vandenbroucke, An Hendrix, Stephan Stremersch, Stefaan C. De Smedt, Koen Raemdonck, Kevin Braeckmans
Format: Article
Language:English
Published: BMC 2025-08-01
Series:Journal of Nanobiotechnology
Online Access:https://doi.org/10.1186/s12951-025-03640-3
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