A Plasmid System That Utilises Phosphoribosylanthranilate Isomerase to Select Against Cells Expressing Truncated Proteins

A Plasmid System That Utilises Phosphoribosylanthranilate Isomerase to Select Against Cells Expressing Truncated Proteins

We have generated a vector that enables the removal of plasmids coding for truncated proteins. This vector expresses a protein of interest in the yeast <i>Saccharomyces cerevisiae</i> from a galactose-inducible promoter. The gene of interest is fused in-frame to a downstream sequence cod...

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Bibliographic Details
Main Authors: Aditi A. Ghuge, Susanne Gottfried, Anja H. Schiemann, Evelyn Sattlegger
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Biomolecules
Subjects:
protein–protein interaction
nonsense mutation
truncated protein
degradation-prone protein
screening
mutagenesis
Online Access:https://www.mdpi.com/2218-273X/15/3/412
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