Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells
Migraine is one of the most common neurological disorders which poses significant socioeconomic burden worldwide. Neuroinflammation and oxidative stress both play important roles in the pathogenesis of migraine. Human urinary kallidinogenase (UK) is a tissue kallikrein derived from human urine. Incr...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Pain Research and Management |
| Online Access: | http://dx.doi.org/10.1155/2019/6393150 |
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| author | Zhongyan Zhao Zhiyu Xu Tao Liu Shixiong Huang Huai Huang Xiaoyun Huang |
| author_facet | Zhongyan Zhao Zhiyu Xu Tao Liu Shixiong Huang Huai Huang Xiaoyun Huang |
| author_sort | Zhongyan Zhao |
| collection | DOAJ |
| description | Migraine is one of the most common neurological disorders which poses significant socioeconomic burden worldwide. Neuroinflammation and oxidative stress both play important roles in the pathogenesis of migraine. Human urinary kallidinogenase (UK) is a tissue kallikrein derived from human urine. Increasing evidence suggests that UK may protect against ischemic stroke, but UK’s treatment potential against migraine remains to be explored. Immortal BV-2 murine microglial cells were treated with UK (125 nM, 250 nM, and 500 nM) and then given lipopolysaccharides (LPS, 1000 ng/mL). Cell viability of BV-2 cells was tested by the CCK-8 assay. Expressions of tumor necrosis factor-α (TNFα), prostaglandin E2 (PGE2), interleukin-6 (IL-6), and interleukin-1β (IL-1β) were examined with the ELISA method and western blot. Intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) were measured to determine oxidative stress. Our results showed that LPS administration increased the levels of proinflammatory cytokines (TNFα, PGE2, IL-6, and IL-1β) and oxidative stress (ROS and MDA) when compared with the control group and decreased significantly upon introduction with UK. Taken together, UK treatment reduced LPS-induced neuroinflammation and oxidative stress in a dose-dependent manner, which might be a potential treatment of migraine. |
| format | Article |
| id | doaj-art-dc2012271c674a9b800130c65758c68b |
| institution | Kabale University |
| issn | 1203-6765 1918-1523 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pain Research and Management |
| spelling | doaj-art-dc2012271c674a9b800130c65758c68b2025-02-03T05:45:17ZengWileyPain Research and Management1203-67651918-15232019-01-01201910.1155/2019/63931506393150Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 CellsZhongyan Zhao0Zhiyu Xu1Tao Liu2Shixiong Huang3Huai Huang4Xiaoyun Huang5Department of Neurology, Hainan General Hospital, Haikou 570311, ChinaDepartment of Critical Care Medicine, Hainan General Hospital, Haikou 570311, ChinaDepartment of Neurology, Hainan General Hospital, Haikou 570311, ChinaDepartment of Neurology, Hainan General Hospital, Haikou 570311, ChinaNeurorehabilitation Dept. 2, Guangzhou General Hospital of Guangzhou Military Command of PLA, Guangzhou 510120, ChinaDepartment of Neurology, The Affiliated Houjie Hospital, Guangdong Medical University, Dongguan 523945, ChinaMigraine is one of the most common neurological disorders which poses significant socioeconomic burden worldwide. Neuroinflammation and oxidative stress both play important roles in the pathogenesis of migraine. Human urinary kallidinogenase (UK) is a tissue kallikrein derived from human urine. Increasing evidence suggests that UK may protect against ischemic stroke, but UK’s treatment potential against migraine remains to be explored. Immortal BV-2 murine microglial cells were treated with UK (125 nM, 250 nM, and 500 nM) and then given lipopolysaccharides (LPS, 1000 ng/mL). Cell viability of BV-2 cells was tested by the CCK-8 assay. Expressions of tumor necrosis factor-α (TNFα), prostaglandin E2 (PGE2), interleukin-6 (IL-6), and interleukin-1β (IL-1β) were examined with the ELISA method and western blot. Intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) were measured to determine oxidative stress. Our results showed that LPS administration increased the levels of proinflammatory cytokines (TNFα, PGE2, IL-6, and IL-1β) and oxidative stress (ROS and MDA) when compared with the control group and decreased significantly upon introduction with UK. Taken together, UK treatment reduced LPS-induced neuroinflammation and oxidative stress in a dose-dependent manner, which might be a potential treatment of migraine.http://dx.doi.org/10.1155/2019/6393150 |
| spellingShingle | Zhongyan Zhao Zhiyu Xu Tao Liu Shixiong Huang Huai Huang Xiaoyun Huang Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells Pain Research and Management |
| title | Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells |
| title_full | Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells |
| title_fullStr | Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells |
| title_full_unstemmed | Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells |
| title_short | Human Urinary Kallidinogenase Reduces Lipopolysaccharide-Induced Neuroinflammation and Oxidative Stress in BV-2 Cells |
| title_sort | human urinary kallidinogenase reduces lipopolysaccharide induced neuroinflammation and oxidative stress in bv 2 cells |
| url | http://dx.doi.org/10.1155/2019/6393150 |
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