Cloning and sequencing of the S gene of H52 strain from avian infectious bronchitis virus
Virion RNA from H52 strain of avian Infectious Bronchitis were extracted from the allantoic fluid of inoculated embryonated eggs and used as template for cDNA synthesis by reverse transcription with 3'-primer. Polymerase chain reaction was performed with two primers which span the S gene, and a...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2002-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2002.03.0303 |
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| Summary: | Virion RNA from H52 strain of avian Infectious Bronchitis were extracted from the allantoic fluid of inoculated embryonated eggs and used as template for cDNA synthesis by reverse transcription with 3'-primer. Polymerase chain reaction was performed with two primers which span the S gene, and an expected product of 3.6 kb was obtained. The PCR product was ligated to pBlueScript SK vector, and its nucleotide sequence was determined by the dideoxy-mediated chain termination method. Comparison and analysis of the nucleotide and deduced amino acid sequence of S gene with that of other IBV strains from GenBank was performed by computer with DNAstar and PCgene software. The results showed that the homology of S gene between H52 and other strains was 83.45%-99.71% on nucleotide level, while 82.10%-99.26% identity was found on amino acid level. Phylogenetic tree of the nucleotide sequence of IBV strain S gene was constructed by computer. |
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| ISSN: | 1008-9209 2097-5155 |