Cloning and sequencing of the S gene of H52 strain from avian infectious bronchitis virus

Virion RNA from H52 strain of avian Infectious Bronchitis were extracted from the allantoic fluid of inoculated embryonated eggs and used as template for cDNA synthesis by reverse transcription with 3'-primer. Polymerase chain reaction was performed with two primers which span the S gene, and a...

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Bibliographic Details
Main Authors: CHENG Li-qin, ZHOU Ji-yong, SHEN Xing-yan, CHEN Ji-gang, CHEN Sheng-ming
Format: Article
Language:English
Published: Zhejiang University Press 2002-05-01
Series:浙江大学学报. 农业与生命科学版
Subjects:
Online Access:https://www.academax.com/doi/10.3785/1008-9209.2002.03.0303
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