UMPlex™: a targeted next-generation sequencing primer design workflow

UMPlex™: a targeted next-generation sequencing primer design workflow

Abstract We have developed a tailored next-generation sequencing (tNGS) panel, employing our innovative UMPlex™ primer design workflow, to enhance pathogen identification in clinical diagnostics. Through iterative experimentation and rigorous validation, we refined the primer design by excluding tho...

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Bibliographic Details
Main Authors: Dachuan Lin, Xiaomin Zhang, Dan Wang, Tongyingzi Liu, Tong Li, Yingluan Zhang, Lihua Li, Yi Huang, Yongchao Guo, Renli Zhang, Xinchun Chen, Tiejian Feng
Format: Article
Language:English
Published: BMC 2025-07-01
Series:Virology Journal
Subjects:
Targeted next-generation sequencing
Personalized primer design
Clinical surveillance
Influenza-like patients
Online Access:https://doi.org/10.1186/s12985-025-02831-6
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https://doi.org/10.1186/s12985-025-02831-6

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