A comprehensive protocol for PDMS fabrication for use in cell culture.

Cells exhibit remarkable sensitivity to the mechanical properties of their surrounding matrix, particularly stiffness changes, a phenomenon known as cellular mechanotransduction. In vivo, tissues exhibit a wide range of stiffness, from kilopascals (kPa) to megapascals (MPa), which can alter with agi...

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Bibliographic Details
Main Authors: Aisling J Greaney, Clíona M McCarthy, Jishnu Padacherri Vethil, Mannthalah Abubaker, Erin C Reardon, Frederick D Crowley, Eoghan M Cunnane, John J E Mulvihill
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2025-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0323283
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