Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner

Abstract Background Regulation of the target DNA cleavage activity of CRISPR/Cas has naturally evolved in a few bacteria or bacteriophages but is lacking in higher species. Thus, identification of bioactive agents and mechanisms that can suppress the activity of Cas9 is urgently needed to rebalance...

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Bibliographic Details
Main Authors: Yuxuan Zhang, Wentao Zou, Yueyang Zhou, Jiaqi Chen, Youtian Hu, Fang Wu
Format: Article
Language:English
Published: BMC 2025-03-01
Series:Genome Biology
Subjects:
Online Access:https://doi.org/10.1186/s13059-025-03521-w
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