Enhancing alfalfa and sorghum silage quality using agricultural wastes: fermentation dynamics, microbial communities, and functional insights
Abstract At present, there are many researches on the nutritional quality and fermentation quality of forage silage by adding distillers’ grain and fruit residue, but few researches on the succession and function prediction of microbiotic community. In this study, the potential of Moutai distillers&...
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| Language: | English |
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BMC
2025-05-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-025-06722-4 |
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| author | Yuanyuan Zhao Dianpeng Liu Yulian Chen Yao Lei Maoya Li Jiachuhan Wang Xiangjiang He Yu Yang Xinyi Zhang Shengnan Liu Xiaoqing Zhang Qiming Cheng Chao Chen |
| author_facet | Yuanyuan Zhao Dianpeng Liu Yulian Chen Yao Lei Maoya Li Jiachuhan Wang Xiangjiang He Yu Yang Xinyi Zhang Shengnan Liu Xiaoqing Zhang Qiming Cheng Chao Chen |
| author_sort | Yuanyuan Zhao |
| collection | DOAJ |
| description | Abstract At present, there are many researches on the nutritional quality and fermentation quality of forage silage by adding distillers’ grain and fruit residue, but few researches on the succession and function prediction of microbiotic community. In this study, the potential of Moutai distillers' grain (MDG), Rosa roxburghii pomace (RP) and Lactobacillus acidophilus (LAB) to improve silage quality during anaerobic storage of alfalfa and sorghum was investigated. Harvested alfalfa and sorghum were ensiled without (CK) or with MDG, RP, LAB, LAB + MDG or LAB + RP for 45 days at 21–25 °C. Compared with the uninoculated control, alfalfa silage inoculated with LAB + MDG presented better nutrient retention, where the lactic acid (LA) content was increased by 84.62% and the ammonia nitrogen (AN) content was reduced by 38.52%. Similarly, in sorghum silage, both inoculation with LAB + MDG and inoculation with LAB + RP effectively increased nutrient retention, increased the LA content and reduced the AN content. The proportion of Lactobacillus increased in sorghum and alfalfa silage after 45 days of fermentation. Inoculation of alfalfa and sorghum with RP or LAB + MDG significantly increased the relative abundance of lactic acid bacteria in silage, especially Lactobacillus plantarum, which was the main dominant strain. The addition of MDG to the feeds not only effectively retained the crude protein (CP) content of the feeds for better retention of their nutritional value but also significantly reduced the contents of neutral detergent fiber (NDF) and acid detergent fiber (ADF), which improved the digestibility and utilization of the feeds. In addition, the addition of MDG further promoted the proliferation of Lactobacillus and increased their abundance in the silage, thus contributing to the improvement of fermentation quality and preservation of silage. In summary, MDG, LAB + MDG, and RP + LAB resulted in higher-quality silage, but the addition of MDG was more cost effective and therefore is recommended for application in production. |
| format | Article |
| id | doaj-art-da9749df1f9a4b31a5f39539062d7f63 |
| institution | OA Journals |
| issn | 1471-2229 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj-art-da9749df1f9a4b31a5f39539062d7f632025-08-20T02:37:59ZengBMCBMC Plant Biology1471-22292025-05-0125111610.1186/s12870-025-06722-4Enhancing alfalfa and sorghum silage quality using agricultural wastes: fermentation dynamics, microbial communities, and functional insightsYuanyuan Zhao0Dianpeng Liu1Yulian Chen2Yao Lei3Maoya Li4Jiachuhan Wang5Xiangjiang He6Yu Yang7Xinyi Zhang8Shengnan Liu9Xiaoqing Zhang10Qiming Cheng11Chao Chen12College of Animal Science, Guizhou UniversityTongliao Academy of Agriculture and Animal Husbandry SciencesCollege of Animal Science, Guizhou UniversityCollege of Animal Science, Guizhou UniversityCollege of Animal Science, Guizhou UniversityCollege of Animal Science, Guizhou UniversityCollege of Animal Science, Guizhou UniversityCollege of Animal Science, Guizhou UniversityCollege of Animal Science, Guizhou UniversityInner Mongolia Forestry and Grassland Monitoring and Planning InstituteGrassland Research of the Chinese Academy of Agricultural SciencesCollege of Animal Science, Guizhou UniversityCollege of Animal Science, Guizhou UniversityAbstract At present, there are many researches on the nutritional quality and fermentation quality of forage silage by adding distillers’ grain and fruit residue, but few researches on the succession and function prediction of microbiotic community. In this study, the potential of Moutai distillers' grain (MDG), Rosa roxburghii pomace (RP) and Lactobacillus acidophilus (LAB) to improve silage quality during anaerobic storage of alfalfa and sorghum was investigated. Harvested alfalfa and sorghum were ensiled without (CK) or with MDG, RP, LAB, LAB + MDG or LAB + RP for 45 days at 21–25 °C. Compared with the uninoculated control, alfalfa silage inoculated with LAB + MDG presented better nutrient retention, where the lactic acid (LA) content was increased by 84.62% and the ammonia nitrogen (AN) content was reduced by 38.52%. Similarly, in sorghum silage, both inoculation with LAB + MDG and inoculation with LAB + RP effectively increased nutrient retention, increased the LA content and reduced the AN content. The proportion of Lactobacillus increased in sorghum and alfalfa silage after 45 days of fermentation. Inoculation of alfalfa and sorghum with RP or LAB + MDG significantly increased the relative abundance of lactic acid bacteria in silage, especially Lactobacillus plantarum, which was the main dominant strain. The addition of MDG to the feeds not only effectively retained the crude protein (CP) content of the feeds for better retention of their nutritional value but also significantly reduced the contents of neutral detergent fiber (NDF) and acid detergent fiber (ADF), which improved the digestibility and utilization of the feeds. In addition, the addition of MDG further promoted the proliferation of Lactobacillus and increased their abundance in the silage, thus contributing to the improvement of fermentation quality and preservation of silage. In summary, MDG, LAB + MDG, and RP + LAB resulted in higher-quality silage, but the addition of MDG was more cost effective and therefore is recommended for application in production.https://doi.org/10.1186/s12870-025-06722-4Lactobacillus acidophilusRosa roxburghii pomaceAlfalfaBacterial communityMoutai distillers' grainSorghum |
| spellingShingle | Yuanyuan Zhao Dianpeng Liu Yulian Chen Yao Lei Maoya Li Jiachuhan Wang Xiangjiang He Yu Yang Xinyi Zhang Shengnan Liu Xiaoqing Zhang Qiming Cheng Chao Chen Enhancing alfalfa and sorghum silage quality using agricultural wastes: fermentation dynamics, microbial communities, and functional insights BMC Plant Biology Lactobacillus acidophilus Rosa roxburghii pomace Alfalfa Bacterial community Moutai distillers' grain Sorghum |
| title | Enhancing alfalfa and sorghum silage quality using agricultural wastes: fermentation dynamics, microbial communities, and functional insights |
| title_full | Enhancing alfalfa and sorghum silage quality using agricultural wastes: fermentation dynamics, microbial communities, and functional insights |
| title_fullStr | Enhancing alfalfa and sorghum silage quality using agricultural wastes: fermentation dynamics, microbial communities, and functional insights |
| title_full_unstemmed | Enhancing alfalfa and sorghum silage quality using agricultural wastes: fermentation dynamics, microbial communities, and functional insights |
| title_short | Enhancing alfalfa and sorghum silage quality using agricultural wastes: fermentation dynamics, microbial communities, and functional insights |
| title_sort | enhancing alfalfa and sorghum silage quality using agricultural wastes fermentation dynamics microbial communities and functional insights |
| topic | Lactobacillus acidophilus Rosa roxburghii pomace Alfalfa Bacterial community Moutai distillers' grain Sorghum |
| url | https://doi.org/10.1186/s12870-025-06722-4 |
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