β-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1
Abstract Background B lymphocytes, essential in cellular immunity as antigen-presenting cells and in humoral immunity as major effector cells, play a crucial role in the antitumor response. Our previous work has shown β-glucan enhanced immunoglobulins (Ig) secretion. But the specific mechanisms of B...
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BMC
2025-01-01
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| Series: | BMC Immunology |
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| Online Access: | https://doi.org/10.1186/s12865-025-00681-z |
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| author | Yu Bai Jun Ding Liuyang He Zhichao Zhu Jie Pan Chunjian Qi |
| author_facet | Yu Bai Jun Ding Liuyang He Zhichao Zhu Jie Pan Chunjian Qi |
| author_sort | Yu Bai |
| collection | DOAJ |
| description | Abstract Background B lymphocytes, essential in cellular immunity as antigen-presenting cells and in humoral immunity as major effector cells, play a crucial role in the antitumor response. Our previous work has shown β-glucan enhanced immunoglobulins (Ig) secretion. But the specific mechanisms of B-cell activation with β-glucan are poorly understood. Here, we took advantage of β-glucan to improve the antitumor immune response of B cells. Results In vitro experiments demonstrate that β-glucan enhance the differentiation of B220lo CD138+ B cells, up-regulate co-stimulatory molecules, and increase the production of cytokines and Ig in response to various antigens. Using the Dectin-1 knockout mice, we revealed that β-glucan modulate B cell immune responses dependent on Dectin-1 receptor. In mouse models of Lewis lung cancer (LLC) tumors, combining β-glucan with programmed death-1(PD-1) blocking antibodies led to increase recruitment of CD19+ B cells in the tumor microenvironment (TME), higher numbers of germinal centers B cells (GC B) in the spleen and draining lymph node (DLN), elevate Ig production, and delay tumor progression. Conclusions These findings reveal that β-glucan can serve as a potent adjuvant to modulate B cell immune responses in a Dectin-1 dependent manner and improve immune checkpoint blockade (ICB) therapy in antitumor. Clinical trial number Not applicable. |
| format | Article |
| id | doaj-art-da87a6e502954f0482ccd61abb3add98 |
| institution | Kabale University |
| issn | 1471-2172 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj-art-da87a6e502954f0482ccd61abb3add982025-01-12T12:14:03ZengBMCBMC Immunology1471-21722025-01-0126111410.1186/s12865-025-00681-zβ-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1Yu Bai0Jun Ding1Liuyang He2Zhichao Zhu3Jie Pan4Chunjian Qi5Laboratory of Oncology, Medical Research Center, The Second People’s Hospital of Changzhou, The Third Affiliated Hospital of Nanjing Medical UniversityLaboratory of Oncology, Medical Research Center, The Second People’s Hospital of Changzhou, The Third Affiliated Hospital of Nanjing Medical UniversityLaboratory of Oncology, Medical Research Center, The Second People’s Hospital of Changzhou, The Third Affiliated Hospital of Nanjing Medical UniversityLaboratory of Oncology, Medical Research Center, The Second People’s Hospital of Changzhou, The Third Affiliated Hospital of Nanjing Medical UniversityLaboratory of Oncology, Medical Research Center, The Second People’s Hospital of Changzhou, The Third Affiliated Hospital of Nanjing Medical UniversityLaboratory of Oncology, Medical Research Center, The Second People’s Hospital of Changzhou, The Third Affiliated Hospital of Nanjing Medical UniversityAbstract Background B lymphocytes, essential in cellular immunity as antigen-presenting cells and in humoral immunity as major effector cells, play a crucial role in the antitumor response. Our previous work has shown β-glucan enhanced immunoglobulins (Ig) secretion. But the specific mechanisms of B-cell activation with β-glucan are poorly understood. Here, we took advantage of β-glucan to improve the antitumor immune response of B cells. Results In vitro experiments demonstrate that β-glucan enhance the differentiation of B220lo CD138+ B cells, up-regulate co-stimulatory molecules, and increase the production of cytokines and Ig in response to various antigens. Using the Dectin-1 knockout mice, we revealed that β-glucan modulate B cell immune responses dependent on Dectin-1 receptor. In mouse models of Lewis lung cancer (LLC) tumors, combining β-glucan with programmed death-1(PD-1) blocking antibodies led to increase recruitment of CD19+ B cells in the tumor microenvironment (TME), higher numbers of germinal centers B cells (GC B) in the spleen and draining lymph node (DLN), elevate Ig production, and delay tumor progression. Conclusions These findings reveal that β-glucan can serve as a potent adjuvant to modulate B cell immune responses in a Dectin-1 dependent manner and improve immune checkpoint blockade (ICB) therapy in antitumor. Clinical trial number Not applicable.https://doi.org/10.1186/s12865-025-00681-zβ-glucanPlasma B cellsDectin-1Tumor immunotherapy |
| spellingShingle | Yu Bai Jun Ding Liuyang He Zhichao Zhu Jie Pan Chunjian Qi β-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1 BMC Immunology β-glucan Plasma B cells Dectin-1 Tumor immunotherapy |
| title | β-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1 |
| title_full | β-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1 |
| title_fullStr | β-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1 |
| title_full_unstemmed | β-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1 |
| title_short | β-Glucan induced plasma B cells differentiation to enhance antitumor immune responses by Dectin-1 |
| title_sort | β glucan induced plasma b cells differentiation to enhance antitumor immune responses by dectin 1 |
| topic | β-glucan Plasma B cells Dectin-1 Tumor immunotherapy |
| url | https://doi.org/10.1186/s12865-025-00681-z |
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