Reduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host Disease
Purpose. To investigate whether the SNARE protein vesicle-associated membrane protein 8 (VAMP8) was implicated in the development of chronic ocular graft-versus-host disease (GVHD). Methods. Firstly, the chronic GVHD (cGVHD) and Sjögren’s syndrome (SS)-impaired lacrimal gland (LG) tissue sections fr...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2017/1639012 |
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| author | Masaki Fukui Yoko Ogawa Shin Mukai Mizuka Kamoi Teru Asato Yutaka Kawakami Kazuo Tsubota |
| author_facet | Masaki Fukui Yoko Ogawa Shin Mukai Mizuka Kamoi Teru Asato Yutaka Kawakami Kazuo Tsubota |
| author_sort | Masaki Fukui |
| collection | DOAJ |
| description | Purpose. To investigate whether the SNARE protein vesicle-associated membrane protein 8 (VAMP8) was implicated in the development of chronic ocular graft-versus-host disease (GVHD). Methods. Firstly, the chronic GVHD (cGVHD) and Sjögren’s syndrome (SS)-impaired lacrimal gland (LG) tissue sections from humans for diagnostic purpose were evaluated for VAMP8 expression by histopathology and immunohistochemistry. Next, serial changes of tear secretion and VAMP8 expression at both protein and mRNA level of LG in an animal cGVHD model compared with the syngeneic control. Results. Decreased VAMP 8 expression in the cGVHD-affected human LG was detected in comparison with SS-affected LG. Tear secretion in the murine cGVHD model was significantly reduced compared with that in the syngeneic controls 8 weeks after BMT. Protein expression of VAMP8 in the cGVHD-affected LG in murine cGVHD was decreased in comparison with that in the controls. Gene expression of VAMP8 in the cGVHD-affected murine LG was significantly less than that in the syngeneic control 3 weeks after BMT. Conclusions. Our results suggested that expression of VAMP8 in the cGVHD-affected LG was decreased and accordingly tear secretion in cGVHD was reduced. Collectively, the reduction of VAMP8 expression in the cGVHD-affected LG can be involved in the pathogenic processes of cGVHD-induced dry eye disease. |
| format | Article |
| id | doaj-art-da330b7e757046d2b15ea54e6f56f854 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-da330b7e757046d2b15ea54e6f56f8542025-02-03T01:09:43ZengWileyJournal of Ophthalmology2090-004X2090-00582017-01-01201710.1155/2017/16390121639012Reduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host DiseaseMasaki Fukui0Yoko Ogawa1Shin Mukai2Mizuka Kamoi3Teru Asato4Yutaka Kawakami5Kazuo Tsubota6Department of Ophthalmology, Keio University School of Medicine, Tokyo, JapanDepartment of Ophthalmology, Keio University School of Medicine, Tokyo, JapanDepartment of Ophthalmology, Keio University School of Medicine, Tokyo, JapanDepartment of Ophthalmology, Keio University School of Medicine, Tokyo, JapanDepartment of Ophthalmology, Keio University School of Medicine, Tokyo, JapanInstitute for Advanced Medical Research, Keio University School of Medicine, Tokyo, JapanDepartment of Ophthalmology, Keio University School of Medicine, Tokyo, JapanPurpose. To investigate whether the SNARE protein vesicle-associated membrane protein 8 (VAMP8) was implicated in the development of chronic ocular graft-versus-host disease (GVHD). Methods. Firstly, the chronic GVHD (cGVHD) and Sjögren’s syndrome (SS)-impaired lacrimal gland (LG) tissue sections from humans for diagnostic purpose were evaluated for VAMP8 expression by histopathology and immunohistochemistry. Next, serial changes of tear secretion and VAMP8 expression at both protein and mRNA level of LG in an animal cGVHD model compared with the syngeneic control. Results. Decreased VAMP 8 expression in the cGVHD-affected human LG was detected in comparison with SS-affected LG. Tear secretion in the murine cGVHD model was significantly reduced compared with that in the syngeneic controls 8 weeks after BMT. Protein expression of VAMP8 in the cGVHD-affected LG in murine cGVHD was decreased in comparison with that in the controls. Gene expression of VAMP8 in the cGVHD-affected murine LG was significantly less than that in the syngeneic control 3 weeks after BMT. Conclusions. Our results suggested that expression of VAMP8 in the cGVHD-affected LG was decreased and accordingly tear secretion in cGVHD was reduced. Collectively, the reduction of VAMP8 expression in the cGVHD-affected LG can be involved in the pathogenic processes of cGVHD-induced dry eye disease.http://dx.doi.org/10.1155/2017/1639012 |
| spellingShingle | Masaki Fukui Yoko Ogawa Shin Mukai Mizuka Kamoi Teru Asato Yutaka Kawakami Kazuo Tsubota Reduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host Disease Journal of Ophthalmology |
| title | Reduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host Disease |
| title_full | Reduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host Disease |
| title_fullStr | Reduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host Disease |
| title_full_unstemmed | Reduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host Disease |
| title_short | Reduced Expression of VAMP8 in Lacrimal Gland Affected by Chronic Graft-versus-Host Disease |
| title_sort | reduced expression of vamp8 in lacrimal gland affected by chronic graft versus host disease |
| url | http://dx.doi.org/10.1155/2017/1639012 |
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