Exogenous Reference RNA for Normalization of Real-Time Quantitative PCR
We have utilized an in vitro transcribed 3′ mRNA fragment of the plant gene ribulose bisphosphate carboxylase (RuBisCO) as an exogenous standard for normalization of quantitative PCR data. Both K562 cells and primary erythroid CD34+ progenitor cells were treated with sodium butyrate and changes in γ...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2003-01-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03341st05 |
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