Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia
Previously, we described a group of patients with hemocytopenia who did not conform to diagnostic criteria of known hematological and nonhematological diseases. Most patients responded well to adrenocortical hormone and/or high-dose intravenous immunoglobulin treatment, indicating that cytopenia mig...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/297678 |
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| author | Hui Liu Rong Fu Yihao Wang Hong Liu Lijuan Li Honglei Wang Jin Chen Hong Yu Zonghong Shao |
| author_facet | Hui Liu Rong Fu Yihao Wang Hong Liu Lijuan Li Honglei Wang Jin Chen Hong Yu Zonghong Shao |
| author_sort | Hui Liu |
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| description | Previously, we described a group of patients with hemocytopenia who did not conform to diagnostic criteria of known hematological and nonhematological diseases. Most patients responded well to adrenocortical hormone and/or high-dose intravenous immunoglobulin treatment, indicating that cytopenia might be mediated by autoantibodies. Autoantibodies were detected on the membrane of various bone marrow (BM) hemopoietic cells by bone marrow mononuclear-cell-Coombs test or flow cytometric analysis. Thus, the hemocytopenia was termed “Immunorelated Pancytopenia” (IRP) to distinguish it from other pancytopenias. Autoantigens in IRP were investigated by membrane protein extraction from BM hemopoietic cells and BM supernatant from IRP patients. Autoantibody IgG was detected in the BM supernatant of 75% of patients (15/20), which was significantly higher than that in aplastic anemia, myelodysplastic syndrome, or autoimmune hemolytic anemia patients (0%) and normal healthy controls (0%) (P<0.01). Autoantigens had approximate molecular weights of 25, 30, 47.5, 60, 65, 70, and 80 kDa, some of which were further identified by mass fingerprinting. This study identified that a G-protein-coupled receptor 156 variant and chain P, a crystal structure of the cytoplasmic domain of human erythrocyte band-3 protein, were autoantigens in IRP. Further studies are needed to confirm the antigenicity of these autoantigens. |
| format | Article |
| id | doaj-art-d999d1c416d04b6aa5bb5e23134c0185 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
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| series | Clinical and Developmental Immunology |
| spelling | doaj-art-d999d1c416d04b6aa5bb5e23134c01852025-02-03T06:14:15ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/297678297678Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated PancytopeniaHui Liu0Rong Fu1Yihao Wang2Hong Liu3Lijuan Li4Honglei Wang5Jin Chen6Hong Yu7Zonghong Shao8Department of Hematology, Tianjin Medical University General Hospital, 154 Anshan Street, Heping District, Tianjin 300052, ChinaDepartment of Hematology, Tianjin Medical University General Hospital, 154 Anshan Street, Heping District, Tianjin 300052, ChinaDepartment of Hematology, Tianjin Medical University General Hospital, 154 Anshan Street, Heping District, Tianjin 300052, ChinaDepartment of Hematology, Tianjin Medical University General Hospital, 154 Anshan Street, Heping District, Tianjin 300052, ChinaDepartment of Hematology, Tianjin Medical University General Hospital, 154 Anshan Street, Heping District, Tianjin 300052, ChinaDepartment of Hematology, Tianjin Medical University General Hospital, 154 Anshan Street, Heping District, Tianjin 300052, ChinaDepartment of Hematology, Tianjin Medical University General Hospital, 154 Anshan Street, Heping District, Tianjin 300052, ChinaDepartment of Hematology, Tianjin Medical University General Hospital, 154 Anshan Street, Heping District, Tianjin 300052, ChinaDepartment of Hematology, Tianjin Medical University General Hospital, 154 Anshan Street, Heping District, Tianjin 300052, ChinaPreviously, we described a group of patients with hemocytopenia who did not conform to diagnostic criteria of known hematological and nonhematological diseases. Most patients responded well to adrenocortical hormone and/or high-dose intravenous immunoglobulin treatment, indicating that cytopenia might be mediated by autoantibodies. Autoantibodies were detected on the membrane of various bone marrow (BM) hemopoietic cells by bone marrow mononuclear-cell-Coombs test or flow cytometric analysis. Thus, the hemocytopenia was termed “Immunorelated Pancytopenia” (IRP) to distinguish it from other pancytopenias. Autoantigens in IRP were investigated by membrane protein extraction from BM hemopoietic cells and BM supernatant from IRP patients. Autoantibody IgG was detected in the BM supernatant of 75% of patients (15/20), which was significantly higher than that in aplastic anemia, myelodysplastic syndrome, or autoimmune hemolytic anemia patients (0%) and normal healthy controls (0%) (P<0.01). Autoantigens had approximate molecular weights of 25, 30, 47.5, 60, 65, 70, and 80 kDa, some of which were further identified by mass fingerprinting. This study identified that a G-protein-coupled receptor 156 variant and chain P, a crystal structure of the cytoplasmic domain of human erythrocyte band-3 protein, were autoantigens in IRP. Further studies are needed to confirm the antigenicity of these autoantigens.http://dx.doi.org/10.1155/2013/297678 |
| spellingShingle | Hui Liu Rong Fu Yihao Wang Hong Liu Lijuan Li Honglei Wang Jin Chen Hong Yu Zonghong Shao Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia Clinical and Developmental Immunology |
| title | Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia |
| title_full | Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia |
| title_fullStr | Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia |
| title_full_unstemmed | Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia |
| title_short | Detection and Analysis of Autoantigens Targeted by Autoantibodies in Immunorelated Pancytopenia |
| title_sort | detection and analysis of autoantigens targeted by autoantibodies in immunorelated pancytopenia |
| url | http://dx.doi.org/10.1155/2013/297678 |
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