Collagen-1 and elastin expression in cervical tissue: A comparison across cervical elongation, pelvic organ prolapse, and combined conditions
HIGHLIGHTS • Pathogenesis of cervical elongation is still limited, including the histological and molecular differences between a cervical elongation and a normal cervix. • The expression of collagen-1 level in the cervical elongation group was stronger compared to the cervical elongation with PO...
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Universitas Airlangga
2024-11-01
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| Series: | Majalah Obstetri dan Ginekologi |
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| Online Access: | https://e-journal.unair.ac.id/MOG/article/view/57883 |
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| author | Anis Widyasari Gatut Hardianto Etty Hari Kusumastuti Eighty Mardiyan Kurniawati |
| author_facet | Anis Widyasari Gatut Hardianto Etty Hari Kusumastuti Eighty Mardiyan Kurniawati |
| author_sort | Anis Widyasari |
| collection | DOAJ |
| description | HIGHLIGHTS
• Pathogenesis of cervical elongation is still limited, including the histological and molecular differences between a cervical elongation and a normal cervix.
• The expression of collagen-1 level in the cervical elongation group was stronger compared to the cervical elongation with POP and control group.
ABSTRACT
Objective: This study aimed to assess differences in the expression of Collagen-1 and Elastin in cervical tissues among patients with Cervical Elongation (CE), Pelvic Organ Prolapse (POP), a combination of CE with POP, and those without either condition.
Materials and Methods: An analytical study with a cross-sectional design was conducted, using immunohistochemistry (IHC) to analyze cervical tissue samples preserved in paraffin blocks. Patient groups included those diagnosed with CE, POP, CE combined with POP, and a control group without CE or POP. All participants underwent surgery between January 2021 and April 2023. IHC was used to measure the expression levels of Collagen-1 and Elastin in each tissue sample. Observations were made under 400x magnification, focusing on five randomly selected visual-field areas in each sample to determine the area fraction. Two experienced pathologists conducted the analyses in a blinded manner to ensure objective evaluation.
Results: Statistical analysis using the Kruskal-Wallis test revealed significant differences in the expression of Collagen-1 across the four groups (CE, POP, CE with POP, and control). Patients with CE showed a higher expression of Collagen-1 than those with CE and POP combined, as well as the control group. However, no significant differences in Elastin expression were observed among the groups.
Conclusion: Collagen-1 expression differs significantly across patients with CE, POP, and CE combined with POP, suggesting a distinct role in cervical tissue remodeling in these conditions. Conversely, Elastin expression was consistent across all groups, indicating that it may not play a differentiating role in these pathologies. These findings highlight Collagen-1’s potential involvement in the structural changes associated with CE and POP. |
| format | Article |
| id | doaj-art-d975681e556247388924ce55d651df1f |
| institution | Kabale University |
| issn | 0854-0381 2598-1013 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Majalah Obstetri dan Ginekologi |
| spelling | doaj-art-d975681e556247388924ce55d651df1f2025-01-07T03:23:16ZengUniversitas AirlanggaMajalah Obstetri dan Ginekologi0854-03812598-10132024-11-0132318919510.20473/mog.V32I32024.189-19556049Collagen-1 and elastin expression in cervical tissue: A comparison across cervical elongation, pelvic organ prolapse, and combined conditionsAnis Widyasari0https://orcid.org/0000-0001-9474-3531Gatut Hardianto1https://orcid.org/0000-0002-5922-5349Etty Hari Kusumastuti2https://orcid.org/0000-0001-7954-9749Eighty Mardiyan Kurniawati3https://orcid.org/0000-0002-7682-9275Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, SurabayaDepartment of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, SurabayaDepartment of Anatomic Pathology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, SurabayaDepartment of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Dr. Soetomo General Academic Hospital, SurabayaHIGHLIGHTS • Pathogenesis of cervical elongation is still limited, including the histological and molecular differences between a cervical elongation and a normal cervix. • The expression of collagen-1 level in the cervical elongation group was stronger compared to the cervical elongation with POP and control group. ABSTRACT Objective: This study aimed to assess differences in the expression of Collagen-1 and Elastin in cervical tissues among patients with Cervical Elongation (CE), Pelvic Organ Prolapse (POP), a combination of CE with POP, and those without either condition. Materials and Methods: An analytical study with a cross-sectional design was conducted, using immunohistochemistry (IHC) to analyze cervical tissue samples preserved in paraffin blocks. Patient groups included those diagnosed with CE, POP, CE combined with POP, and a control group without CE or POP. All participants underwent surgery between January 2021 and April 2023. IHC was used to measure the expression levels of Collagen-1 and Elastin in each tissue sample. Observations were made under 400x magnification, focusing on five randomly selected visual-field areas in each sample to determine the area fraction. Two experienced pathologists conducted the analyses in a blinded manner to ensure objective evaluation. Results: Statistical analysis using the Kruskal-Wallis test revealed significant differences in the expression of Collagen-1 across the four groups (CE, POP, CE with POP, and control). Patients with CE showed a higher expression of Collagen-1 than those with CE and POP combined, as well as the control group. However, no significant differences in Elastin expression were observed among the groups. Conclusion: Collagen-1 expression differs significantly across patients with CE, POP, and CE combined with POP, suggesting a distinct role in cervical tissue remodeling in these conditions. Conversely, Elastin expression was consistent across all groups, indicating that it may not play a differentiating role in these pathologies. These findings highlight Collagen-1’s potential involvement in the structural changes associated with CE and POP.https://e-journal.unair.ac.id/MOG/article/view/57883cervical elongationpelvic organ prolapsecollagen-1elastinmaternal health |
| spellingShingle | Anis Widyasari Gatut Hardianto Etty Hari Kusumastuti Eighty Mardiyan Kurniawati Collagen-1 and elastin expression in cervical tissue: A comparison across cervical elongation, pelvic organ prolapse, and combined conditions Majalah Obstetri dan Ginekologi cervical elongation pelvic organ prolapse collagen-1 elastin maternal health |
| title | Collagen-1 and elastin expression in cervical tissue: A comparison across cervical elongation, pelvic organ prolapse, and combined conditions |
| title_full | Collagen-1 and elastin expression in cervical tissue: A comparison across cervical elongation, pelvic organ prolapse, and combined conditions |
| title_fullStr | Collagen-1 and elastin expression in cervical tissue: A comparison across cervical elongation, pelvic organ prolapse, and combined conditions |
| title_full_unstemmed | Collagen-1 and elastin expression in cervical tissue: A comparison across cervical elongation, pelvic organ prolapse, and combined conditions |
| title_short | Collagen-1 and elastin expression in cervical tissue: A comparison across cervical elongation, pelvic organ prolapse, and combined conditions |
| title_sort | collagen 1 and elastin expression in cervical tissue a comparison across cervical elongation pelvic organ prolapse and combined conditions |
| topic | cervical elongation pelvic organ prolapse collagen-1 elastin maternal health |
| url | https://e-journal.unair.ac.id/MOG/article/view/57883 |
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