Protocol for minicircle production for gene therapy without subsequent cleanup steps

Protocol for minicircle production for gene therapy without subsequent cleanup steps

Summary: Current techniques for producing minicircles have low yields because of the cleanup steps required. Here, we present a protocol that can increase minicircle yield up to 10-fold by eliminating post-induction cleanup steps. We describe steps for bacterial culture, same-day arabinose induction...

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Bibliographic Details
Main Authors: Jonathan Do, Gautam Verma, Yasmin Maurice, Muniba Abdumanobova, Zicheng Deng, Tanya V. Kalin, Vladimir V. Kalinichenko
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Biotechnology and bioengineering
Gene Expression
Microbiology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003880
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http://www.sciencedirect.com/science/article/pii/S2666166725003880

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