Affinity Tag for Protein Purification and Detection Based on the Disulfide-Linked Complex of InaD and NorpA

Affinity tags are not only used for the expression and purification of recombinant proteins but also for the detection of protein-protein interactions. Common problems with many affinity tags are excessive length, which may interfere with the structure and function of tagged proteins, and low affini...

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Bibliographic Details
Main Authors: Michelle E. Kimple, John Sondek
Format: Article
Language:English
Published: Taylor & Francis Group 2002-09-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/02333rr01
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