Precise measurement of molecular phenotypes with barcode-based CRISPRi systems

Abstract Genome-wide CRISPR-Cas9 screens have untangled regulatory networks driving diverse biological processes. Their success relies on interrogating specific molecular phenotypes and distinguishing key regulators from background effects. Here, we realize these goals by optimizing CRISPR interfere...

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Bibliographic Details
Main Authors: Joseph H. Lobel, Nicholas T. Ingolia
Format: Article
Language:English
Published: BMC 2025-05-01
Series:Genome Biology
Subjects:
Online Access:https://doi.org/10.1186/s13059-025-03610-w
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