Oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis model
Background: Porphyromonas gingivalis is a key pathogen in periodontitis, which involves inflammation and alveolar bone loss. An imbalance of cytokines and growth factors contributes to disease progression. Rice husk liquid smoke has shown potential antibacterial and anti-inflammatory properties. Obj...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-11-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426825001800 |
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| author | Gustiadi Saputra Ira Arundina Theresia Indah Budhy Dieni Mansur Meircurius Dwi Condro Surboyo Cheng Hwee Ming Sheryn Marcha Ramaniasari Azzahra Salsabila Adira Moelyanto |
| author_facet | Gustiadi Saputra Ira Arundina Theresia Indah Budhy Dieni Mansur Meircurius Dwi Condro Surboyo Cheng Hwee Ming Sheryn Marcha Ramaniasari Azzahra Salsabila Adira Moelyanto |
| author_sort | Gustiadi Saputra |
| collection | DOAJ |
| description | Background: Porphyromonas gingivalis is a key pathogen in periodontitis, which involves inflammation and alveolar bone loss. An imbalance of cytokines and growth factors contributes to disease progression. Rice husk liquid smoke has shown potential antibacterial and anti-inflammatory properties. Objective: This study aimed to formulate and characterize an oral thin film (OTF) containing rice husk liquid smoke and evaluate its antibacterial activity and biological effects in a periodontitis model. Methods: OTFs were prepared using hydroxypropyl methylcellulose, gelatin, sorbitol, and rice husk liquid smoke via solvent casting. The OTF were characterized for weight, thickness, swelling, disintegration, dissolution, and antibacterial activity against Porphyromonas gingivalis. For in vivo testing, periodontitis was induced in 28 Wistar rats. OTFs were applied to periodontal pockets. Mandibular tissues were collected on days 2 and 7 for immunohistochemical analysis of IL-6, IL-10, FGF-2, and BMP-2 expression. Results: The OTFs showed favorable physical properties, including low weight, appropriate thickness, and faster disintegration and dissolution times (p < 0.05). Both OTF and blank films inhibited Porphyromonas gingivalis growth. OTF treatment significantly decreased IL-6 and increased IL-10, FGF-2, and BMP-2 expression at both time points (p < 0.05), indicating reduced inflammation and enhanced tissue regeneration. Conclusion: The OTF containing rice husk liquid smoke exhibited strong antibacterial and immunomodulatory effects, suggesting its potential as a novel therapeutic strategy for periodontitis. |
| format | Article |
| id | doaj-art-d9348e32309245f59d6b06c2d0d14755 |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2025-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-d9348e32309245f59d6b06c2d0d147552025-08-20T03:41:40ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-11-011561266127310.1016/j.jobcr.2025.08.007Oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis modelGustiadi Saputra0Ira Arundina1Theresia Indah Budhy2Dieni Mansur3Meircurius Dwi Condro Surboyo4Cheng Hwee Ming5Sheryn Marcha Ramaniasari6Azzahra Salsabila Adira Moelyanto7Politeknik Kesehatan Kesuma Bangsa, Bandar Lampung, IndonesiaDepartment of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia; Corresponding author. Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, East Java, Indonesia.Department of Oral Pathology and Maxillofacial, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, IndonesiaResearch Center for Chemistry, National Research and Innovation Agency, B.J. Habibie Science and Technopark, Serpong, South Tangerang 15314, Banten, IndonesiaDepartment of Oral Medicine, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, Indonesia; Corresponding author. Department of Oral Medicine, Faculty of Dental Medicine, Universitas AirlanggaJalan Prof. Dr. Moestopo No 47, Surabaya, 60132, Indonesia.Department Physiology. Faculty of Medicine. University of Malaya, Kuala Lumpur, 50603, MalaysiaMagister of Dental Health Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, IndonesiaMagister of Dental Health Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, 60132, IndonesiaBackground: Porphyromonas gingivalis is a key pathogen in periodontitis, which involves inflammation and alveolar bone loss. An imbalance of cytokines and growth factors contributes to disease progression. Rice husk liquid smoke has shown potential antibacterial and anti-inflammatory properties. Objective: This study aimed to formulate and characterize an oral thin film (OTF) containing rice husk liquid smoke and evaluate its antibacterial activity and biological effects in a periodontitis model. Methods: OTFs were prepared using hydroxypropyl methylcellulose, gelatin, sorbitol, and rice husk liquid smoke via solvent casting. The OTF were characterized for weight, thickness, swelling, disintegration, dissolution, and antibacterial activity against Porphyromonas gingivalis. For in vivo testing, periodontitis was induced in 28 Wistar rats. OTFs were applied to periodontal pockets. Mandibular tissues were collected on days 2 and 7 for immunohistochemical analysis of IL-6, IL-10, FGF-2, and BMP-2 expression. Results: The OTFs showed favorable physical properties, including low weight, appropriate thickness, and faster disintegration and dissolution times (p < 0.05). Both OTF and blank films inhibited Porphyromonas gingivalis growth. OTF treatment significantly decreased IL-6 and increased IL-10, FGF-2, and BMP-2 expression at both time points (p < 0.05), indicating reduced inflammation and enhanced tissue regeneration. Conclusion: The OTF containing rice husk liquid smoke exhibited strong antibacterial and immunomodulatory effects, suggesting its potential as a novel therapeutic strategy for periodontitis.http://www.sciencedirect.com/science/article/pii/S2212426825001800Oral thin filmLiquid smokePeriodontitisPorphyromonas gingivalisInflammation |
| spellingShingle | Gustiadi Saputra Ira Arundina Theresia Indah Budhy Dieni Mansur Meircurius Dwi Condro Surboyo Cheng Hwee Ming Sheryn Marcha Ramaniasari Azzahra Salsabila Adira Moelyanto Oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis model Journal of Oral Biology and Craniofacial Research Oral thin film Liquid smoke Periodontitis Porphyromonas gingivalis Inflammation |
| title | Oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis model |
| title_full | Oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis model |
| title_fullStr | Oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis model |
| title_full_unstemmed | Oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis model |
| title_short | Oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis model |
| title_sort | oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis model |
| topic | Oral thin film Liquid smoke Periodontitis Porphyromonas gingivalis Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S2212426825001800 |
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