T7 DNA polymerase treatment improves quantitative sequencing of both double-stranded and single-stranded DNA viruses

T7 DNA polymerase treatment improves quantitative sequencing of both double-stranded and single-stranded DNA viruses

Bulk microbiome, as well as virome-enriched shotgun sequencing only reveals the double-stranded DNA (dsDNA) content of a given sample, unless specific treatments are applied. However, genomes of viruses often consist of a circular single-stranded DNA (ssDNA) molecule. Pre-treatment and amplification...

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Bibliographic Details
Main Authors: Billaud, Maud, Theodorou, Ilias, Lamy-Besnier, Quentin, Shah, Shiraz A., Lecointe, François, De Sordi, Luisa, De Paepe, Marianne, Petit, Marie-Agnès
Format: Article
Language:English
Published: Peer Community In 2024-07-01
Series:Peer Community Journal
Subjects:
T7 DNA polymerase
shotgun sequencing
single-stranded DNA
Online Access:https://peercommunityjournal.org/articles/10.24072/pcjournal.437/
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