T7 DNA polymerase treatment improves quantitative sequencing of both double-stranded and single-stranded DNA viruses
Bulk microbiome, as well as virome-enriched shotgun sequencing only reveals the double-stranded DNA (dsDNA) content of a given sample, unless specific treatments are applied. However, genomes of viruses often consist of a circular single-stranded DNA (ssDNA) molecule. Pre-treatment and amplification...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Peer Community In
2024-07-01
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| Series: | Peer Community Journal |
| Subjects: | |
| Online Access: | https://peercommunityjournal.org/articles/10.24072/pcjournal.437/ |
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