Preparation and Characterization of Liquid Fertilizers Produced by Anaerobic Fermentation

Biol is a liquid product, obtained by anaerobic fermentation of local inputs, which improves the health of agroecosystems, which is an emerging area in agronomy. The aim of this study consists of the preparation of two biols from inoculums of cow dung (BCD) and native forest duff (BNF) by using spec...

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Main Authors: Juan Francisco López-Rubio, Cristina Cebrián-Tarancón, Gonzalo L. Alonso, Maria Rosario Salinas, Rosario Sánchez-Gómez
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Agriculture
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Online Access:https://www.mdpi.com/2077-0472/15/11/1225
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author Juan Francisco López-Rubio
Cristina Cebrián-Tarancón
Gonzalo L. Alonso
Maria Rosario Salinas
Rosario Sánchez-Gómez
author_facet Juan Francisco López-Rubio
Cristina Cebrián-Tarancón
Gonzalo L. Alonso
Maria Rosario Salinas
Rosario Sánchez-Gómez
author_sort Juan Francisco López-Rubio
collection DOAJ
description Biol is a liquid product, obtained by anaerobic fermentation of local inputs, which improves the health of agroecosystems, which is an emerging area in agronomy. The aim of this study consists of the preparation of two biols from inoculums of cow dung (BCD) and native forest duff (BNF) by using specific biodigesters and commercial inputs. The biol characterization was made in terms of mineral (ionic and complex forms), amino acids, hormones and volatile compounds, along with Pfeiffer circular chromatography during fermentation monitoring. The results showed a pH acidic in both biols (4.5–5.5), which is higher for BCD. Also, this biol had higher content in several macro- and micronutrients in ionic (nitrates, phosphates, calcium, iron and sodium) and complex forms (calcium, iron and potassium). Both have interesting content in amino acids and hormones. The absence of microorganisms in the final products could be due to the presence of volatile compounds such as pyrazines and sulfoxides. Along with this, other volatile compounds such as esters were identified, which can be responsible for their pleasant odor. The novelty of this work is to provide a protocol for obtaining biols and to demonstrate their potential to be used as biofertilizers.
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institution OA Journals
issn 2077-0472
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publishDate 2025-06-01
publisher MDPI AG
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spelling doaj-art-d80033da2bbf4980b063159be154cf722025-08-20T02:23:43ZengMDPI AGAgriculture2077-04722025-06-011511122510.3390/agriculture15111225Preparation and Characterization of Liquid Fertilizers Produced by Anaerobic FermentationJuan Francisco López-Rubio0Cristina Cebrián-Tarancón1Gonzalo L. Alonso2Maria Rosario Salinas3Rosario Sánchez-Gómez4Cátedra de Química Agrícola, E.T.S. de Ingeniería Agronómica y de Montes y Biotecnología, Universidad de Castilla-La Mancha, Avda. de España s/n, 02071 Albacete, SpainCátedra de Química Agrícola, E.T.S. de Ingeniería Agronómica y de Montes y Biotecnología, Universidad de Castilla-La Mancha, Avda. de España s/n, 02071 Albacete, SpainCátedra de Química Agrícola, E.T.S. de Ingeniería Agronómica y de Montes y Biotecnología, Universidad de Castilla-La Mancha, Avda. de España s/n, 02071 Albacete, SpainCátedra de Química Agrícola, E.T.S. de Ingeniería Agronómica y de Montes y Biotecnología, Universidad de Castilla-La Mancha, Avda. de España s/n, 02071 Albacete, SpainCátedra de Química Agrícola, E.T.S. de Ingeniería Agronómica y de Montes y Biotecnología, Universidad de Castilla-La Mancha, Avda. de España s/n, 02071 Albacete, SpainBiol is a liquid product, obtained by anaerobic fermentation of local inputs, which improves the health of agroecosystems, which is an emerging area in agronomy. The aim of this study consists of the preparation of two biols from inoculums of cow dung (BCD) and native forest duff (BNF) by using specific biodigesters and commercial inputs. The biol characterization was made in terms of mineral (ionic and complex forms), amino acids, hormones and volatile compounds, along with Pfeiffer circular chromatography during fermentation monitoring. The results showed a pH acidic in both biols (4.5–5.5), which is higher for BCD. Also, this biol had higher content in several macro- and micronutrients in ionic (nitrates, phosphates, calcium, iron and sodium) and complex forms (calcium, iron and potassium). Both have interesting content in amino acids and hormones. The absence of microorganisms in the final products could be due to the presence of volatile compounds such as pyrazines and sulfoxides. Along with this, other volatile compounds such as esters were identified, which can be responsible for their pleasant odor. The novelty of this work is to provide a protocol for obtaining biols and to demonstrate their potential to be used as biofertilizers.https://www.mdpi.com/2077-0472/15/11/1225amino acidsbiolcircular chromatographycow dungforest duffhormones
spellingShingle Juan Francisco López-Rubio
Cristina Cebrián-Tarancón
Gonzalo L. Alonso
Maria Rosario Salinas
Rosario Sánchez-Gómez
Preparation and Characterization of Liquid Fertilizers Produced by Anaerobic Fermentation
Agriculture
amino acids
biol
circular chromatography
cow dung
forest duff
hormones
title Preparation and Characterization of Liquid Fertilizers Produced by Anaerobic Fermentation
title_full Preparation and Characterization of Liquid Fertilizers Produced by Anaerobic Fermentation
title_fullStr Preparation and Characterization of Liquid Fertilizers Produced by Anaerobic Fermentation
title_full_unstemmed Preparation and Characterization of Liquid Fertilizers Produced by Anaerobic Fermentation
title_short Preparation and Characterization of Liquid Fertilizers Produced by Anaerobic Fermentation
title_sort preparation and characterization of liquid fertilizers produced by anaerobic fermentation
topic amino acids
biol
circular chromatography
cow dung
forest duff
hormones
url https://www.mdpi.com/2077-0472/15/11/1225
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AT gonzalolalonso preparationandcharacterizationofliquidfertilizersproducedbyanaerobicfermentation
AT mariarosariosalinas preparationandcharacterizationofliquidfertilizersproducedbyanaerobicfermentation
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