Identification of Metabolites in Lima Bean (Phaseolus lunatus) Flour Using Gas Chromatography-Mass Spectrometry (GC-MS)

Lima bean (Phaseolus lunatus) is an underutilized legume, rich in nutrients and other beneficial constituents that can enhance health and has the potential to contribute to food security and nutritional well-being globally. Gas chromatography-mass spectrometry (GC-MS) analysis was used to profile th...

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Main Author: Janet Adebo
Format: Article
Language:English
Published: Hasan Eleroğlu 2025-07-01
Series:Turkish Journal of Agriculture: Food Science and Technology
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Online Access:https://agrifoodscience.com/index.php/TURJAF/article/view/7607
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author Janet Adebo
author_facet Janet Adebo
author_sort Janet Adebo
collection DOAJ
description Lima bean (Phaseolus lunatus) is an underutilized legume, rich in nutrients and other beneficial constituents that can enhance health and has the potential to contribute to food security and nutritional well-being globally. Gas chromatography-mass spectrometry (GC-MS) analysis was used to profile the metabolites of Lima beans (Phaseolus lunatus) led to the identification of 47 metabolites. The group of compounds detected with their percentage levels are alcohol (4%), amino acids (8%), ester (4%), fatty acids (11%), glycoside (2%), inorganic acid (2%), miscellaneous compounds (4%), nitrogenous compounds (11%), organic acids (15%), sugars (30%), and sugar alcohols (9%). Sugars were the main metabolite group in the Lima bean, with sucrose having the highest average peak areas and total average area percentage levels in this group. Fructose, glucose, galactose, maltose, cellobiose and their different forms were also present. Fatty acids such as stearic and palmitic acids were identified, this indicating that Lima beans could serve as a healthy source of fat for humans and as fodder. This study demonstrated that Lima beans contain important metabolites of health and nutritional importance that could support their inclusion in a healthy diet and improve their utilization in food.
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spelling doaj-art-d74625018e8448e88b8b8d5e33158b302025-08-20T02:46:35ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2025-07-011371707171210.24925/turjaf.v13i7.1707-1712.76076308Identification of Metabolites in Lima Bean (Phaseolus lunatus) Flour Using Gas Chromatography-Mass Spectrometry (GC-MS)Janet Adebo0https://orcid.org/0000-0003-0044-9126Centre for Cyber-Physical Food, Energy and Water Systems (CCP-FEWS), University of Johannesburg, South Africa.Lima bean (Phaseolus lunatus) is an underutilized legume, rich in nutrients and other beneficial constituents that can enhance health and has the potential to contribute to food security and nutritional well-being globally. Gas chromatography-mass spectrometry (GC-MS) analysis was used to profile the metabolites of Lima beans (Phaseolus lunatus) led to the identification of 47 metabolites. The group of compounds detected with their percentage levels are alcohol (4%), amino acids (8%), ester (4%), fatty acids (11%), glycoside (2%), inorganic acid (2%), miscellaneous compounds (4%), nitrogenous compounds (11%), organic acids (15%), sugars (30%), and sugar alcohols (9%). Sugars were the main metabolite group in the Lima bean, with sucrose having the highest average peak areas and total average area percentage levels in this group. Fructose, glucose, galactose, maltose, cellobiose and their different forms were also present. Fatty acids such as stearic and palmitic acids were identified, this indicating that Lima beans could serve as a healthy source of fat for humans and as fodder. This study demonstrated that Lima beans contain important metabolites of health and nutritional importance that could support their inclusion in a healthy diet and improve their utilization in food.https://agrifoodscience.com/index.php/TURJAF/article/view/7607legume cropbioactive compoundsmethanol extractionmetabolitesprofilinghealth benefits
spellingShingle Janet Adebo
Identification of Metabolites in Lima Bean (Phaseolus lunatus) Flour Using Gas Chromatography-Mass Spectrometry (GC-MS)
Turkish Journal of Agriculture: Food Science and Technology
legume crop
bioactive compounds
methanol extraction
metabolites
profiling
health benefits
title Identification of Metabolites in Lima Bean (Phaseolus lunatus) Flour Using Gas Chromatography-Mass Spectrometry (GC-MS)
title_full Identification of Metabolites in Lima Bean (Phaseolus lunatus) Flour Using Gas Chromatography-Mass Spectrometry (GC-MS)
title_fullStr Identification of Metabolites in Lima Bean (Phaseolus lunatus) Flour Using Gas Chromatography-Mass Spectrometry (GC-MS)
title_full_unstemmed Identification of Metabolites in Lima Bean (Phaseolus lunatus) Flour Using Gas Chromatography-Mass Spectrometry (GC-MS)
title_short Identification of Metabolites in Lima Bean (Phaseolus lunatus) Flour Using Gas Chromatography-Mass Spectrometry (GC-MS)
title_sort identification of metabolites in lima bean phaseolus lunatus flour using gas chromatography mass spectrometry gc ms
topic legume crop
bioactive compounds
methanol extraction
metabolites
profiling
health benefits
url https://agrifoodscience.com/index.php/TURJAF/article/view/7607
work_keys_str_mv AT janetadebo identificationofmetabolitesinlimabeanphaseoluslunatusflourusinggaschromatographymassspectrometrygcms