Assessment of Mathematical Approaches for the Estimation and Comparison of Efficiency in qPCR Assays for a Prokaryotic Model

Assessment of Mathematical Approaches for the Estimation and Comparison of Efficiency in qPCR Assays for a Prokaryotic Model

Quantitative PCR is a molecular technique for DNA quantification that depends on reaction efficiency and the Ct value (“cycle threshold”). However, the results are dependent on laboratory conditions and mathematical approaches. Thus, the data of 16 genes from <i>Pseudomonas aeruginosa</i>...

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Bibliographic Details
Main Authors: Jose Arturo Molina-Mora, Meriyeins Sibaja-Amador, Luis Rivera-Montero, Daniel Chacón-Arguedas, Caterina Guzmán, Fernando García
Format: Article
Language:English
Published: MDPI AG 2024-06-01
Series:DNA
Subjects:
qPCR
efficiency
mathematical model
gene expression
DNA quantification
Online Access:https://www.mdpi.com/2673-8856/4/3/12
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