Phytochemical Characterization Utilizing HS-SPME/GC-MS: Exploration of the Antioxidant and Enzyme Inhibition Properties of Essential Oil from Saudi <i>Artemisia absinthium</i> L.
<b>Background/Objectives:</b> This study aimed to analyze the chemical composition and biological activities of <i>Artemisia absinthium</i> L. essential oil, focusing on its antioxidant and enzyme inhibition (α-amylase and urease) properties. Additionally, in vitro pharmacoki...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Pharmaceuticals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8247/17/11/1460 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850068187523055616 |
|---|---|
| author | Hanan Y. Aati Hala A. Attia Arwa S. Alanazi Luluh K. AL tamran Juergen K. Wanner |
| author_facet | Hanan Y. Aati Hala A. Attia Arwa S. Alanazi Luluh K. AL tamran Juergen K. Wanner |
| author_sort | Hanan Y. Aati |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> This study aimed to analyze the chemical composition and biological activities of <i>Artemisia absinthium</i> L. essential oil, focusing on its antioxidant and enzyme inhibition (α-amylase and urease) properties. Additionally, in vitro pharmacokinetic and pharmacodynamic evaluations were conducted through in silico molecular docking and BOILED-Egg models to assess its therapeutic potential and its potency in treating oxidative-stress-related diseases. <b>Methods:</b> The essential oil was isolated by the hydrodistillation (HD) of fresh plant material, and volatiles released from dried plant material were sampled via headspace solid-phase microextraction (HS-SPME), followed by a phytochemical profiling analysis through the GC-MS tool. Antioxidant capacity was assessed using DPPH, ABTS, FRAP, and nitric oxide scavenging assays, while enzyme inhibition activities were tested against α-amylase and urease. Molecular docking and BOILED-Egg models were used to evaluate compound interactions with NADPH oxidase and predict pharmacokinetic behavior, respectively. <b>Results:</b> HS-SPME and HD yielded 46 and 25 compounds, respectively, primarily terpenoids represented by camphor (26.4%) and cis-davanone (18.0%) in HS-SPME, while in the HD essential oil, cis-davanone (60.2%) and chamazulene (10.8%) were most prevalent. The antioxidant assays showed a strong activity, with a total antioxidant capacity of 821.8 mg ascorbic acid Eq/gm. The essential oil inhibited urease by 86.7% and α-amylase by 81.8%. Molecular docking showed strong binding affinities with NADPH oxidase, supporting the antioxidant results. <b>Conclusions:</b><i>A. absinthium</i> EO demonstrated potent antioxidant and enzyme inhibitory activities, suggesting its therapeutic potential for treating enzyme-related disorders like diabetes mellitus and its possible use as a cure for many oxidative-stress-related diseases, thus validating the folkloric use of this plant. |
| format | Article |
| id | doaj-art-d6f8b8e82f514f1f970fb8ee77cb632b |
| institution | DOAJ |
| issn | 1424-8247 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-d6f8b8e82f514f1f970fb8ee77cb632b2025-08-20T02:48:07ZengMDPI AGPharmaceuticals1424-82472024-10-011711146010.3390/ph17111460Phytochemical Characterization Utilizing HS-SPME/GC-MS: Exploration of the Antioxidant and Enzyme Inhibition Properties of Essential Oil from Saudi <i>Artemisia absinthium</i> L.Hanan Y. Aati0Hala A. Attia1Arwa S. Alanazi2Luluh K. AL tamran3Juergen K. Wanner4Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaCollege of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaCollege of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi ArabiaKurt Kitzing Co., Hinterm Alten Schloss 21, D-86757 Wallerstein, Germany<b>Background/Objectives:</b> This study aimed to analyze the chemical composition and biological activities of <i>Artemisia absinthium</i> L. essential oil, focusing on its antioxidant and enzyme inhibition (α-amylase and urease) properties. Additionally, in vitro pharmacokinetic and pharmacodynamic evaluations were conducted through in silico molecular docking and BOILED-Egg models to assess its therapeutic potential and its potency in treating oxidative-stress-related diseases. <b>Methods:</b> The essential oil was isolated by the hydrodistillation (HD) of fresh plant material, and volatiles released from dried plant material were sampled via headspace solid-phase microextraction (HS-SPME), followed by a phytochemical profiling analysis through the GC-MS tool. Antioxidant capacity was assessed using DPPH, ABTS, FRAP, and nitric oxide scavenging assays, while enzyme inhibition activities were tested against α-amylase and urease. Molecular docking and BOILED-Egg models were used to evaluate compound interactions with NADPH oxidase and predict pharmacokinetic behavior, respectively. <b>Results:</b> HS-SPME and HD yielded 46 and 25 compounds, respectively, primarily terpenoids represented by camphor (26.4%) and cis-davanone (18.0%) in HS-SPME, while in the HD essential oil, cis-davanone (60.2%) and chamazulene (10.8%) were most prevalent. The antioxidant assays showed a strong activity, with a total antioxidant capacity of 821.8 mg ascorbic acid Eq/gm. The essential oil inhibited urease by 86.7% and α-amylase by 81.8%. Molecular docking showed strong binding affinities with NADPH oxidase, supporting the antioxidant results. <b>Conclusions:</b><i>A. absinthium</i> EO demonstrated potent antioxidant and enzyme inhibitory activities, suggesting its therapeutic potential for treating enzyme-related disorders like diabetes mellitus and its possible use as a cure for many oxidative-stress-related diseases, thus validating the folkloric use of this plant.https://www.mdpi.com/1424-8247/17/11/1460essential oilHS-SPMEHDenzyme inhibitionsNADPH oxidaseGC-MS |
| spellingShingle | Hanan Y. Aati Hala A. Attia Arwa S. Alanazi Luluh K. AL tamran Juergen K. Wanner Phytochemical Characterization Utilizing HS-SPME/GC-MS: Exploration of the Antioxidant and Enzyme Inhibition Properties of Essential Oil from Saudi <i>Artemisia absinthium</i> L. Pharmaceuticals essential oil HS-SPME HD enzyme inhibitions NADPH oxidase GC-MS |
| title | Phytochemical Characterization Utilizing HS-SPME/GC-MS: Exploration of the Antioxidant and Enzyme Inhibition Properties of Essential Oil from Saudi <i>Artemisia absinthium</i> L. |
| title_full | Phytochemical Characterization Utilizing HS-SPME/GC-MS: Exploration of the Antioxidant and Enzyme Inhibition Properties of Essential Oil from Saudi <i>Artemisia absinthium</i> L. |
| title_fullStr | Phytochemical Characterization Utilizing HS-SPME/GC-MS: Exploration of the Antioxidant and Enzyme Inhibition Properties of Essential Oil from Saudi <i>Artemisia absinthium</i> L. |
| title_full_unstemmed | Phytochemical Characterization Utilizing HS-SPME/GC-MS: Exploration of the Antioxidant and Enzyme Inhibition Properties of Essential Oil from Saudi <i>Artemisia absinthium</i> L. |
| title_short | Phytochemical Characterization Utilizing HS-SPME/GC-MS: Exploration of the Antioxidant and Enzyme Inhibition Properties of Essential Oil from Saudi <i>Artemisia absinthium</i> L. |
| title_sort | phytochemical characterization utilizing hs spme gc ms exploration of the antioxidant and enzyme inhibition properties of essential oil from saudi i artemisia absinthium i l |
| topic | essential oil HS-SPME HD enzyme inhibitions NADPH oxidase GC-MS |
| url | https://www.mdpi.com/1424-8247/17/11/1460 |
| work_keys_str_mv | AT hananyaati phytochemicalcharacterizationutilizinghsspmegcmsexplorationoftheantioxidantandenzymeinhibitionpropertiesofessentialoilfromsaudiiartemisiaabsinthiumil AT halaaattia phytochemicalcharacterizationutilizinghsspmegcmsexplorationoftheantioxidantandenzymeinhibitionpropertiesofessentialoilfromsaudiiartemisiaabsinthiumil AT arwasalanazi phytochemicalcharacterizationutilizinghsspmegcmsexplorationoftheantioxidantandenzymeinhibitionpropertiesofessentialoilfromsaudiiartemisiaabsinthiumil AT luluhkaltamran phytochemicalcharacterizationutilizinghsspmegcmsexplorationoftheantioxidantandenzymeinhibitionpropertiesofessentialoilfromsaudiiartemisiaabsinthiumil AT juergenkwanner phytochemicalcharacterizationutilizinghsspmegcmsexplorationoftheantioxidantandenzymeinhibitionpropertiesofessentialoilfromsaudiiartemisiaabsinthiumil |